Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

In Vitro Multiplication of Lophostemon suaveolens (Sol.ex Gaertn.) Peter G.Wilson & J.T. Waterh): Peatland Tree Species for Rehabilitations

Asri Insiana Putri
* ORCID logo ,
Noor Khomsah Kartikawati
,
Arif Nirsatmanto
,
Sri Sunarti
,
Liliek Haryjanto
,
Toni Herawan
,
Purwanto Budi Santosa
,
Reni Setyo Wahyuningtyas
,
Fajar Lestari
,
Anto Rimbawanto
Version 1 : Received: 4 October 2022 / Approved: 24 October 2022 / Online: 24 October 2022 (03:31:08 CEST)

A peer-reviewed article of this Preprint also exists.

Putri, A.I.; Kartikawati, N.K.; Nirsatmanto, A.; Sunarti, S.; Haryjanto, L.; Herawan, T.; Santosa, P.B.; Wahyuningtyas, R.S.; Lestari, F.; Rimbawanto, A. In Vitro Multiplication of Lophostemon suaveolens (Sol.ex Gaertn.) Peter G.Wilson & J.T. Waterh): Peatland Tree Species for Rehabilitation. Sustainability 2022, 14, 14720. Putri, A.I.; Kartikawati, N.K.; Nirsatmanto, A.; Sunarti, S.; Haryjanto, L.; Herawan, T.; Santosa, P.B.; Wahyuningtyas, R.S.; Lestari, F.; Rimbawanto, A. In Vitro Multiplication of Lophostemon suaveolens (Sol.ex Gaertn.) Peter G.Wilson & J.T. Waterh): Peatland Tree Species for Rehabilitation. Sustainability 2022, 14, 14720.

Abstract

Peatlands in Indonesia are one of the world's largest carbon sinks, helping to regulate greenhouse gas emissions and global climate change. Lophostemon suaveolens is a plant found in Papua's endemic peat ecosystem that grows well in wet areas with low fertility. It is geographically dispersed and has the potential for peatland re-habilitation. We present an efficient and reproducible protocol for in vitro multiplication of plantlets using nodal segments and shoot apices collected from plantlets. The Murashige and Skoog (MS) medium was sup-plemented with single and double kinds of PGRs (BAP and NAA). The concentration used in this study is based on the best findings of earlier preliminary research on single PGR treatment (0.5 ml/l BAP and 0.1 ml/l NAA) for the initiation stage. After 3 months of in vitro axillary shoot initiation, elongated shoots from plant-lets were separated from the clumps and multiplied. Until 6 months incubation, the potential multiplication of axillary shoots was determined by counting the number of nodule multiplication coefficients (NMC), shoot length, root length, and number of leaves (6 successive subculture). The highest average per plantlet of NMC (8.4 0.13) was observed in MS medium with a combination of 0.5 ml/l BAP and 0.1 ml/l NAA (double PGRs), with the longest shoots (5.91 0.17 cm), the longest root length (8.83 0.69 cm), and the most leaves (32 0.69). They were cultured in a pH 5 environment with a light photoperiod (40 mol/m2/s intensity) for 16 hours. When BAP and NAA were used together after acclimatization, seedling survival was highest. This is the first report of L. suaveolens in vitro multiplication, and the protocol could be used to propagate this peatland species on a large scale.

Keywords

Lophostemon suaveolens; peatland rehabilitation; in vitro multiplication; PGRs; acclimatization

Subject

Biology and Life Sciences, Biology and Biotechnology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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