Version 1
: Received: 19 October 2022 / Approved: 20 October 2022 / Online: 20 October 2022 (08:25:43 CEST)
How to cite:
Fazliana, M.; Ahmad Zamri, L.; Khan, H. Differential Expression of Insulin Resistance Genes in Polycystic Ovary Syndrome. Preprints2022, 2022100299. https://doi.org/10.20944/preprints202210.0299.v1
Fazliana, M.; Ahmad Zamri, L.; Khan, H. Differential Expression of Insulin Resistance Genes in Polycystic Ovary Syndrome. Preprints 2022, 2022100299. https://doi.org/10.20944/preprints202210.0299.v1
Fazliana, M.; Ahmad Zamri, L.; Khan, H. Differential Expression of Insulin Resistance Genes in Polycystic Ovary Syndrome. Preprints2022, 2022100299. https://doi.org/10.20944/preprints202210.0299.v1
APA Style
Fazliana, M., Ahmad Zamri, L., & Khan, H. (2022). Differential Expression of Insulin Resistance Genes in Polycystic Ovary Syndrome. Preprints. https://doi.org/10.20944/preprints202210.0299.v1
Chicago/Turabian Style
Fazliana, M., Liyana Ahmad Zamri and Hanifullah Khan. 2022 "Differential Expression of Insulin Resistance Genes in Polycystic Ovary Syndrome" Preprints. https://doi.org/10.20944/preprints202210.0299.v1
Abstract
Background Polycystic ovary syndrome (PCOS) is a common hormonal disorder worldwide among women of reproductive age. It is characterized by endocrine, reproductive, and metabolic abnormalities. Insulin resistance (IR) is one of its most important clinical features, associated with metabolic disorders and increased risk of type 2 diabetes (T2D). This study aimed to explore the whole blood gene expression profiling related to IR in PCOS patients compared to controls. Methods Blood RNA was extracted from 5 PCOS and 5 non-PCOS women with matched age and BMI. Homeostasis model assessment (HOMA-IR) was used to estimate the IR. The expression of IR genes was analyzed by Profiler PCR array. Results Both groups have similar levels of HOMA-IR (p>0.05). However, differential expression levels were observed between them. Fourteen genes were upregulated and 26 genes were downregulated in PCOS samples. Among the upregulated genes (>2 fold-change, p-value<0.05) are ADIPOQ, ADIPOR1, OLR, IGF-1, and APOE. Downregulated genes (>-2 fold-change, p-value<0.05) include HK-2, IRS1, and SERPINE1. These genes are involved in insulin and adipokines signaling, commonly dysregulated in T2D. They are also involved in innate immunity and inflammatory processes and are essential for lipid and carbohydrate metabolism. Conclusion Our finding suggests that despite both groups having no difference in IR level, there are differentially expressed genes involved in the IR pathway.
Medicine and Pharmacology, Obstetrics and Gynaecology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
