Preprint Review Version 1 Preserved in Portico This version is not peer-reviewed

Study of Cell Migration under Mechanical Confinement in Microfluidic Lab-on-a-Chip

Version 1 : Received: 20 June 2022 / Approved: 22 June 2022 / Online: 22 June 2022 (08:21:44 CEST)

A peer-reviewed article of this Preprint also exists.

Sala, F.; Ficorella, C.; Osellame, R.; Käs, J.A.; Martínez Vázquez, R. Microfluidic Lab-on-a-Chip for Studies of Cell Migration under Spatial Confinement. Biosensors 2022, 12, 604. Sala, F.; Ficorella, C.; Osellame, R.; Käs, J.A.; Martínez Vázquez, R. Microfluidic Lab-on-a-Chip for Studies of Cell Migration under Spatial Confinement. Biosensors 2022, 12, 604.

Journal reference: Biosensors 2022, 12, 604
DOI: 10.3390/bios12080604

Abstract

Understanding cell migration is a key step to unravel many physiological phenomena and predict several pathologies, like cancer metastasis. In particular, mechanical confinement has been proved to be a key factor in the cellular migration strategy choice. As our insight in the field improves, new tools are needed in order to empower biologists’ analysis capabilities. In this framework, microfluidic devices have been used to engineer the mechanical stimuli and to investigate cellular migration response in a more controlled way. In this work, we will review the existing technologies employed in the realization of microfluidic cellular migration assays, namely soft lithography of PDMS and hydrogels and femtosecond laser micromachining. We will give an overview of the state of the art of these devices, focusing on the different geometrical configurations that have been exploited to study specific aspects of cellular migration. Our scope is to highlight the advantages and possibilities given by each approach and to envisage the future developments in in-vitro migration studies under mechanical confinement in microfluidic devices.

Keywords

lab-on-a-chip; cell migration; microfluidics; PDMS; hydrogels; femtosecond laser microfabrication; two-photon polymerization

Subject

LIFE SCIENCES, Biophysics

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