Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Effect of Fermentation Response on Biosynthesis of Endopolygalacturonase by Utilizing Polymeric Substrates of Agricultural Origin from a Potent Strain of Bacillus

Nagina Rafique
ORCID logo ,
Romana Tabassum
,
Muhammad Zubair Khan
* ,
Tawaf Ali Shah
,
Saiqa Bashir
,
Saima Rafique
,
Imran Hayat
,
Imtiaz Hussain
,
Shahzad Akbar
ORCID logo ,
Khawaja Shafique Ahmad
Version 1 : Received: 30 May 2022 / Approved: 1 June 2022 / Online: 1 June 2022 (09:58:48 CEST)

How to cite: Rafique, N.; Tabassum, R.; Khan, M.Z.; Shah, T.A.; Bashir, S.; Rafique, S.; Hayat, I.; Hussain, I.; Akbar, S.; Ahmad, K.S. Effect of Fermentation Response on Biosynthesis of Endopolygalacturonase by Utilizing Polymeric Substrates of Agricultural Origin from a Potent Strain of Bacillus. Preprints 2022, 2022060012. https://doi.org/10.20944/preprints202206.0012.v1 Rafique, N.; Tabassum, R.; Khan, M.Z.; Shah, T.A.; Bashir, S.; Rafique, S.; Hayat, I.; Hussain, I.; Akbar, S.; Ahmad, K.S. Effect of Fermentation Response on Biosynthesis of Endopolygalacturonase by Utilizing Polymeric Substrates of Agricultural Origin from a Potent Strain of Bacillus. Preprints 2022, 2022060012. https://doi.org/10.20944/preprints202206.0012.v1

Abstract

Endopolygalacturonase (EndoPGase) is one of the crucial pectinases belonging to the class of carbohydrase. The catalytic action of EndoPGase captivate the attention for production of this extremely valuable catalyst of industrial sector. The main focus was to ascertain a potential bacterial candidate for endoPGase production. The isolated bacterial strain was further identified by 16S rDNA gene sequencing. A genomic library was constructed by using Lambda ZAP II vector system to investigate the pectinolytic potential of the expressed genes. The parameters for enzyme biosynthesis were optimized by single as well as multiple factor approach at a time. The results of our investigation led to the identification of a potent strain of Bacillus subtilis NR2. The strain was found active for pectic enzyme activity under shaking- flask fermentation at pH 5.0 and 50 °C temperature of incubation. Among all monomeric and polymeric substrates, citrus pectin followed by wheat bran was considered the best enzyme inducers at 1 % concentration. Moreover, an increasing trend in enzyme activity was observed with the increasing inducer concentration. The combined effect of three variables (pH, substrates, and substrate concentration) was explored by response surface methodology involving Box Benken Design (BBD). The study concluded that the soil isolated B. subtilis can be utilized for commercial scale pectinase enzyme production.

Keywords

Endopolygalacturonase; biocatalysts; biosynthesis; heteropolysaccharide; inducer; submerged fermentation

Subject

Biology and Life Sciences, Agricultural Science and Agronomy

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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