Hernandez-Garcia, A.; Morales-Moreno, M.D.; Valdés-Galindo, E.G.; Jimenez-Nieto, E.P.; Quezada, A. Diagnostics of COVID-19 Based on CRISPR–Cas Coupled to Isothermal Amplification: A Comparative Analysis and Update. Diagnostics2022, 12, 1434.
Hernandez-Garcia, A.; Morales-Moreno, M.D.; Valdés-Galindo, E.G.; Jimenez-Nieto, E.P.; Quezada, A. Diagnostics of COVID-19 Based on CRISPR–Cas Coupled to Isothermal Amplification: A Comparative Analysis and Update. Diagnostics 2022, 12, 1434.
Hernandez-Garcia, A.; Morales-Moreno, M.D.; Valdés-Galindo, E.G.; Jimenez-Nieto, E.P.; Quezada, A. Diagnostics of COVID-19 Based on CRISPR–Cas Coupled to Isothermal Amplification: A Comparative Analysis and Update. Diagnostics2022, 12, 1434.
Hernandez-Garcia, A.; Morales-Moreno, M.D.; Valdés-Galindo, E.G.; Jimenez-Nieto, E.P.; Quezada, A. Diagnostics of COVID-19 Based on CRISPR–Cas Coupled to Isothermal Amplification: A Comparative Analysis and Update. Diagnostics 2022, 12, 1434.
Abstract
The emergence of the COVID-19 pandemics prompted a fast development of novel diagnostic methods of the etiologic virus SARS-CoV-2. Methods based on CRISPR-Cas systems have been particularly promising because they can achieve a similar sensitivity and specificity to the golden standard RT-qPCR, especially when coupled to an isothermal pre-amplification step. Furthermore, they have also solved inherent limitations of RT-qPCR that impede its decentralized use and deployment in the field, such as the need for expensive equipment, high cost per reaction, and delivery of results in hours, among others. In this review, we evaluate publicly available methods to detect SARS-CoV-2 that are based on CRISPR-Cas and isothermal amplification. We critically analyze the steps required to obtain a successful result from clinical samples and pinpoint key experimental conditions and parameters that could be optimized or modified to improve clinical and analytical outputs. The COVID outbreak has propelled intensive research in a short time, which is paving the way to develop effective and very promising CRISPR-Cas systems for the precise detection of SARS-CoV-2. This review could also serve as an introductory guide to new labs delving into this technology.
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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