Version 1
: Received: 11 November 2021 / Approved: 12 November 2021 / Online: 12 November 2021 (17:09:40 CET)
How to cite:
Malec, A.; Haiden, C.; Kokkinis, G.; Giouroudi, I. Microfluidic Platform combined with a Dark Field Imaging System for Quantification of E. coli Contamination in Water. Preprints2021, 2021110240 (doi: 10.20944/preprints202111.0240.v1).
Malec, A.; Haiden, C.; Kokkinis, G.; Giouroudi, I. Microfluidic Platform combined with a Dark Field Imaging System for Quantification of E. coli Contamination in Water. Preprints 2021, 2021110240 (doi: 10.20944/preprints202111.0240.v1).
Cite as:
Malec, A.; Haiden, C.; Kokkinis, G.; Giouroudi, I. Microfluidic Platform combined with a Dark Field Imaging System for Quantification of E. coli Contamination in Water. Preprints2021, 2021110240 (doi: 10.20944/preprints202111.0240.v1).
Malec, A.; Haiden, C.; Kokkinis, G.; Giouroudi, I. Microfluidic Platform combined with a Dark Field Imaging System for Quantification of E. coli Contamination in Water. Preprints 2021, 2021110240 (doi: 10.20944/preprints202111.0240.v1).
Abstract
In this paper, we present a method for detecting and quantifying pathogens in water samples. The method proposes a portable dark field imaging and analysis system for quantifying E. coli concentrations in water after being labeled with magnetic particles. The system utilizes the tracking of moving micro/nano objects close to or below the optical resolution limit confined in small sample volumes (~ 10 µl). In particular, the system analyzes the effect of volumetric changes due to bacteria conjugation to magnetic microparticles (MP) on their Brownian motion while being suspended in liquid buffer solution. The method allows for a simple inexpensive implementation and the possibility to be used as point-of-need testing system. Indeed, a work-ing prototype is demonstrated with the capacity of quantifying E. coli colony forming units (CFU) at a range of 1x10³ - 6x10³ CFU/mL.
Keywords
pathogen detection, bacteria quantification, dark field imaging, hydrodynamic diameter, E.coli, biosensing, water contamination, magnetic microparticles
Subject
ENGINEERING, Biomedical & Chemical Engineering
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
