Version 1
: Received: 17 October 2021 / Approved: 18 October 2021 / Online: 18 October 2021 (14:30:50 CEST)
How to cite:
Zhu, Y.; Zhang, X.; Li, G.; Xiang, J.; Su, J.; Wu, L.; Daud, M.K.; Mei, L. The Complete Chloroplast Genome Sequence of Four Plant Species, Their SSR Identification and Phylogenetic Analysis. Preprints2021, 2021100246. https://doi.org/10.20944/preprints202110.0246.v1
Zhu, Y.; Zhang, X.; Li, G.; Xiang, J.; Su, J.; Wu, L.; Daud, M.K.; Mei, L. The Complete Chloroplast Genome Sequence of Four Plant Species, Their SSR Identification and Phylogenetic Analysis. Preprints 2021, 2021100246. https://doi.org/10.20944/preprints202110.0246.v1
Zhu, Y.; Zhang, X.; Li, G.; Xiang, J.; Su, J.; Wu, L.; Daud, M.K.; Mei, L. The Complete Chloroplast Genome Sequence of Four Plant Species, Their SSR Identification and Phylogenetic Analysis. Preprints2021, 2021100246. https://doi.org/10.20944/preprints202110.0246.v1
APA Style
Zhu, Y., Zhang, X., Li, G., Xiang, J., Su, J., Wu, L., Daud, M.K., & Mei, L. (2021). The Complete Chloroplast Genome Sequence of Four Plant Species, Their SSR Identification and Phylogenetic Analysis. Preprints. https://doi.org/10.20944/preprints202110.0246.v1
Chicago/Turabian Style
Zhu, Y., Muhammad Khan Daud and Lei Mei. 2021 "The Complete Chloroplast Genome Sequence of Four Plant Species, Their SSR Identification and Phylogenetic Analysis" Preprints. https://doi.org/10.20944/preprints202110.0246.v1
Abstract
The chloroplast genome is conservative and stable, which can be employed to resolve genotypes. Currently, published nuclear sequences and molecular markers failed to differentiate the species from taxa robustly, including Machilus leptophylla, Hanceola exserta, Rubus bambusarum, and Rubus henryi. In this study, the four chloroplast genomes were characterized, and then their simple sequence repeats (SSRs) and phylogenetic positions were analyzed. The results demonstrated the four chloroplast genomes consisted of 152.624 kb, 153.296kb, 156.309 kb, and 158.953 kb in length, involving 124, 130, 129, and 131 genes, respectively. Moreover, the chloroplast genomes contained typical four regions. Six classes of SSR were identified from the four chloroplast genomes, in which mononucleotide was the class with the most members. The types of the repeats were various within individual classes of SSR. Phylogenetic trees indicated that M. leptophylla was clustered with M. yunnanensis, and H. exserta was confirmed under family Ocimeae. Additionally, R. bambusarum and R. henryi were clustered together, whereas they did not belong to the same species due to the differing SSR features. This research would provide evidence for resolving the species and contributed new genetic information for further study.
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
