Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Gene Profile Analysis and Molecular-Physiological Evaluation of Tomato Genotypes Under Drought Stress

Version 1 : Received: 2 October 2021 / Approved: 4 October 2021 / Online: 4 October 2021 (15:01:07 CEST)

How to cite: Ghanbari Moheb Seraj, R.; Tohidfar, M.; Ahmadikhah, A. Gene Profile Analysis and Molecular-Physiological Evaluation of Tomato Genotypes Under Drought Stress. Preprints 2021, 2021100062 (doi: 10.20944/preprints202110.0062.v1). Ghanbari Moheb Seraj, R.; Tohidfar, M.; Ahmadikhah, A. Gene Profile Analysis and Molecular-Physiological Evaluation of Tomato Genotypes Under Drought Stress. Preprints 2021, 2021100062 (doi: 10.20944/preprints202110.0062.v1).

Abstract

Identification of the differentially-expressed genes is important for clarification of the complex molecular mechanisms under drought conditions. In this experiment, transcriptome profiles of sensitive and tolerant tomato genotypes under drought stress were analyzed. Three up-regulated genes were selected, included CAB3 (Chlorophyll a-b binding protein3), SAMDC (S-adenosylmethionine decarboxylase proenzyme), and ACS9 (1-aminocyclopropane-1-carboxylate synthase 9). After bioinformatics analysis, tomato genotypes were subjected to drought stress and gene expression was determined using Real-Time-PCR. Physiological parameters of genotypes were also measured by spectrophotometer-based methods. According to the results, these three genes play a key role in stress tolerance. Expression of the CAB3 gene in both sensitive and tolerant genotypes was not significantly different compared to the control, but the SAMDC gene decreased in both genotypes and the ACS9 gene decreased in sensitive genotype and increased in tolerant genotype. The physiological analysis also showed that under stress conditions, the photosynthetic system of the plant was disrupted and the chlorophyll content was reduced, but, proline content and antioxidant enzymes activity increased, in which their quantity in the tolerant genotype was significantly higher than sensitive. Under drought stress, due to damage to the lipid membrane, Malondialdehyde content also increased, in which the sensitive genotype was more affected.

Keywords

Bioinformatics analysis; Drought stress; Gene expression; Physiological parameters; Tomato; Transcriptome.

Comments (0)

We encourage comments and feedback from a broad range of readers. See criteria for comments and our diversity statement.

Leave a public comment
Send a private comment to the author(s)
Views 0
Downloads 0
Comments 0
Metrics 0


×
Alerts
Notify me about updates to this article or when a peer-reviewed version is published.
We use cookies on our website to ensure you get the best experience.
Read more about our cookies here.