Review
Version 2
Preserved in Portico This version is not peer-reviewed
RNAi Crop Protection Advances
Version 1
: Received: 30 September 2021 / Approved: 4 October 2021 / Online: 4 October 2021 (14:20:15 CEST)
Version 2 : Received: 5 October 2021 / Approved: 6 October 2021 / Online: 6 October 2021 (11:48:50 CEST)
Version 3 : Received: 11 October 2021 / Approved: 13 October 2021 / Online: 13 October 2021 (15:39:34 CEST)
Version 2 : Received: 5 October 2021 / Approved: 6 October 2021 / Online: 6 October 2021 (11:48:50 CEST)
Version 3 : Received: 11 October 2021 / Approved: 13 October 2021 / Online: 13 October 2021 (15:39:34 CEST)
A peer-reviewed article of this Preprint also exists.
Hernández-Soto, A.; Chacón-Cerdas, R. RNAi Crop Protection Advances. Int. J. Mol. Sci. 2021, 22, 12148. Hernández-Soto, A.; Chacón-Cerdas, R. RNAi Crop Protection Advances. Int. J. Mol. Sci. 2021, 22, 12148.
Abstract
RNAi technology is a versatile, effective, safe, and eco-friendly alternative for crop protection. There is plenty of evidence of its use through Host-induced gene silencing (HIGS) and spray-induced gene silencing (SIGS) techniques to control viruses, bacteria, fungi, insects, and nematodes. As for SIGS, its most significant challenge is achieving stability and avoiding premature degradation of RNAi in the environment or during its absorption in the target organism. One alternative is the encapsulation in liposomes, virus-like particles, polyplex nanoparticles, and bio-clay, which can be obtained through the recombinant production of RNAi in vectors, transgenesis, and micro/nanoencapsulation. The materials must be safe, biodegradable, and stable in multiple chemical environments favoring the controlled release of RNAi. Most of the current research of encapsulated RNAi focuses primarily on oral delivery to control insects by silencing essential genes. The regulation of RNAi technology focuses on risk assessment from different approaches; however, this technology has positive characteristics for its use in agriculture from the economic, environmental, and human health implications. The emergence of alternatives combining RNAi gene silencing with the induction of resistance in crops by elicitation and metabolic control is expected, as well as multiple silencing and biotechnological optimization of its large-scale production.
Keywords
RNAi; dsRNA; silencing; encapsulation; liposomes; virus-like particles; polyplex nanoparticles; bio-clay; regulatory
Subject
Biology and Life Sciences, Plant Sciences
Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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Commenter: Alejandro Hernandez
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