Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Mushroom’s Evaluation Based on FT-IR Fingerprint and Chemometrics

Version 1 : Received: 24 September 2021 / Approved: 28 September 2021 / Online: 28 September 2021 (21:36:05 CEST)

A peer-reviewed article of this Preprint also exists.

Feher, I.; Floare-Avram, C.V.; Covaciu, F.-D.; Marincas, O.; Puscas, R.; Magdas, D.A.; Sârbu, C. Evaluation of Mushrooms Based on FT-IR Fingerprint and Chemometrics. Appl. Sci. 2021, 11, 9577. Feher, I.; Floare-Avram, C.V.; Covaciu, F.-D.; Marincas, O.; Puscas, R.; Magdas, D.A.; Sârbu, C. Evaluation of Mushrooms Based on FT-IR Fingerprint and Chemometrics. Appl. Sci. 2021, 11, 9577.

Journal reference: Appl. Sci. 2021, 11, 9577
DOI: 10.3390/app11209577

Abstract

Edible mushrooms have been recognized as highly nutritional food for a long time, due to their specific flavor, texture and also for therapeutic effects. This study proposes a new simple approach, based on FT-IR analysis, followed by statistical methods, in order to differentiate three wild mushrooms species from Romanian spontaneous flora, namely Armillaria mellea, Boletus edulis and Cantharellus cibarius. The preliminary data treatment consisted of data set reduction with principal component analysis (PCA), which provided scores for the next methods. Linear discriminant analysis (LDA) manage to 100% classify the three species and the cross validation step of the method returned 97.4% of correctly classified samples. Only one A. mellea sample overlapped on B. edulis group. When kNN was used in the same manner as LDA, the overall percent of correctly classified samples from the training step was 86.21%, while for holdout set the percent raised at 94.74%. The lowered values obtained for the training set was due to one C. cibarius sample, two B. edulis and five A. mellea, which were placed to other species. Anyway, for holdout sample set, only one sample from B. edulis was misclassified. The fuzzy c-means clustering (FCM) analysis successfully classified investigated mushroom samples according to their species, meaning that in every partition the predominant specie had the biggest DOMs, while samples belonging to other specie had lower DOMs.

Keywords

mushrooms; FT-IR; chemometric; machine learning

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