Shurygin, B.; Chivkunova, O.; Solovchenko, O.; Solovchenko, A.; Dorokhov, A.; Smirnov, I.; Astashev, M.E.; Khort, D. Comparison of the Non-Invasive Monitoring of Fresh-Cut Lettuce Condition with Imaging Reflectance Hyperspectrometer and Imaging PAM-Fluorimeter. Photonics2021, 8, 425.
Shurygin, B.; Chivkunova, O.; Solovchenko, O.; Solovchenko, A.; Dorokhov, A.; Smirnov, I.; Astashev, M.E.; Khort, D. Comparison of the Non-Invasive Monitoring of Fresh-Cut Lettuce Condition with Imaging Reflectance Hyperspectrometer and Imaging PAM-Fluorimeter. Photonics 2021, 8, 425.
We compared two approaches to non-invasive proximal sensing of the early changes in fresh-cut lettuce leaf quality: hyperspectral imaging and imaging PAM-fluorometry of chlorophyll contained in the leaves. The assessments made by the imaging techniques were confronted with the quality assessments made by traditional biochemical assays: relative water content and foliar pigment (chlorophyll and carotenoid) composition. The hyperspectral imaging-based approach provided the highest sensitivity to the decline of fresh-cut lettuce leaf quality taking place within 24 h from cutting. Using of the imaging PAM was complicated by (i) weak correlation of the spatial distribution pattern of the Qy parameter with the actual physiological condition of the plant object and (ii) its high degree of heterogeneity. Accordingly, the imaging PAM-based approach was sensitive only to the manifestations of leaf quality degradation only at advanced stages of the process. Sealing the leaves in the polyethylene bags slowed down the leaf quality degradation at the initial stages (< 3 days) but promoted its rate at more advanced stages, likely due to build-up of ethylene in the bags. An approach was developed to the processing of hyperspectral data for non-invasive monitoring of the lettuce leaves with a potential for implementation in greenhouses and packinghouses.
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