Preprint Brief Report Version 1 Preserved in Portico This version is not peer-reviewed

Broadly Neutralizing Anti- HIV-1 Antibodies Do Not Inhibit HIV-1 Env-Mediated Cell-Cell Fusion

Version 1 : Received: 1 September 2021 / Approved: 2 September 2021 / Online: 2 September 2021 (12:13:53 CEST)

How to cite: Düzgüneş, N.; Yee, M.; Chau, D. Broadly Neutralizing Anti- HIV-1 Antibodies Do Not Inhibit HIV-1 Env-Mediated Cell-Cell Fusion. Preprints 2021, 2021090038 (doi: 10.20944/preprints202109.0038.v1). Düzgüneş, N.; Yee, M.; Chau, D. Broadly Neutralizing Anti- HIV-1 Antibodies Do Not Inhibit HIV-1 Env-Mediated Cell-Cell Fusion. Preprints 2021, 2021090038 (doi: 10.20944/preprints202109.0038.v1).

Abstract

PG9, PG16, PGT121, and PGT145 antibodies were identified from culture media of activated memory B-cells of an infected donor and shown to neutralize many HIV-1 strains. Since HIV-1 spreads via both free virions and cell-cell fusion, we examined the effect of the antibodies on HIV-1 Env-mediated cell-cell fusion. Clone69TRevEnv cells that express Env in the absence of tetracycline were labeled with Calcein-AM Green, and incubated with CD4+ SupT1 cells labeled with CellTrace™ Calcein Red-Orange, with or without antibodies. Monoclonal antibodies PG9, PG16, 2G12, PGT121, and PGT145 (at up to 50 µg/mL) had little or no inhibitory effect on fusion between HIV-Env and SupT1 cells. By contrast, Hippeastrum hybrid agglutinin completely inhibited fusion. Our results indicate that transmission of the virus or viral genetic material would not be inhibited by these broadly neutralizing antibodies. Thus, antibodies generated by HIV-1 vaccines should be screened for their inhibitory effect on Env-mediated cell-cell fusion.

Keywords

Membrane fusion; gp120; gp41; bnAb; CD4; virus-cell fusion; syncytium formation

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