Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Construction of a Novel Chimeric Dextransucrase Fused to the Carbohydrate-Binding Module CBM2a

Reinaldo Fraga Vidal
* ORCID logo ,
Roberto Carlos Aristicas Ribalta
,
Lisandra Teresa Martínez Valdés
,
Meinardo Lafargue Gámez
,
Amanda Montes Alvarez
,
Arianne Rubio Sánchez
,
Eric Dubreucq
ORCID logo ,
Benoit Moreau
Version 1 : Received: 30 August 2021 / Approved: 1 September 2021 / Online: 1 September 2021 (12:12:00 CEST)

A peer-reviewed article of this Preprint also exists.

Fraga Vidal, R.; Arísticas Ribalta, R.C.; Martínez Valdés, L.T.; Lafargue Gámez, M.; Montes Alvarez, A.; Rubio Sánchez, A.; Dubreucq, E.; Moreau, B. Construction of a Novel Chimeric Dextransucrase Fused to the Carbohydrate-Binding Module CBM2a. Catalysts 2021, 11, 1179. Fraga Vidal, R.; Arísticas Ribalta, R.C.; Martínez Valdés, L.T.; Lafargue Gámez, M.; Montes Alvarez, A.; Rubio Sánchez, A.; Dubreucq, E.; Moreau, B. Construction of a Novel Chimeric Dextransucrase Fused to the Carbohydrate-Binding Module CBM2a. Catalysts 2021, 11, 1179.

Abstract

The lactic acid bacteria (LAB) have great potential to produce homoexopolysaccharides (HoPS), have been the subject of extensive research efforts, given their health benefits and physicochemical properties. The HoPS functional properties are determined by structural characteristics of varied molecular weights, types of glycosidic linkages, degrees of branching and chemical composition. The dextransucrases (DSases) are responsible of the synthesis of a kind of HoPS (dextran polymers), which are among the first biopolymers produced at industrial scale with applications in medicine and biotechnology. The concept of glycodiversification opens additional applications for DSases. In that sense the design and characterization of new DSases is of prime importance. Previously, we described the isolation and characterization of a novel extracellular dextransucrase (DSR-F) encoding gene. In this study, from DSR-F, we design a novel chimeric dextransucrase DSR-F-∆SP-∆GBD-CBM2a, where DSR-F-∆SP-∆GBD is fused to the carbohydrate-binding module (CBM2a) of the β-1-4 exoglucanase/xylanase Cex (Xyn10A) of Cellulomonas fimi ATCC 484. This dextransucrase variant is active and without alteration in its specificity. The DSR-F-∆SP-∆GBD-CBM2a is purified by cellulose affinity chromatography for the very first time. Our results indicate that new hybrids and chimeric DSases with novel binding capacity to cellulose can be designed to obtain glyco-biocatalysts from renewable lignocellulosic materials.

Keywords

dextransucrases; GH70; lactic acid bacteria; sucrose-active enzymes; carbohydrate binding module; glucansucrase; cellulose binding domain; Leuconostoc

Subject

Biology and Life Sciences, Biochemistry and Molecular Biology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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