Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Total Aseptization of Boar Semen, to Increase the Biosecurity of Reproduction in Swine

Version 1 : Received: 31 August 2021 / Approved: 1 September 2021 / Online: 1 September 2021 (11:52:42 CEST)

A peer-reviewed article of this Preprint also exists.

Ciornei, Ș.; Drugociu, D.; Ciornei, L.M.; Mareș, M.; Roşca, P. Total Aseptization of Boar Semen, to Increase the Biosecurity of Reproduction in Swine. Molecules 2021, 26, 6183. Ciornei, Ș.; Drugociu, D.; Ciornei, L.M.; Mareș, M.; Roşca, P. Total Aseptization of Boar Semen, to Increase the Biosecurity of Reproduction in Swine. Molecules 2021, 26, 6183.

Journal reference: Molecules 2021, 26, 6183
DOI: 10.3390/molecules26206183

Abstract

The aim of the study is to establish the complete microbiological profile of boar semen and to choose the most effective antiseptic measures in order to control and optimize AI reproduction in pigs. More than one hundred semen samples were collected and analyzed from several pig farms. The microbiological profile of ejaculates was determined by determining the degree of con-tamination of fresh semen and after dilution with specific extenders. The bacterial and fungal load of fresh boar semen recorded an average value of 82.41/0.149x103CFU/mL, while after diluting the ejaculates the contamination value was 0.354/0.140 x103CFU/mL. 23 bacterial and fungal species were isolated, the most common being Candida parapsilosis/sake (92%), Escherichia coli (81.2%). Modification of the sperm collection protocol (HPBC) reduced contamination in raw sperm (by 49.85% in bacteria and by 9.67% in fungi). The load in bacteria and filamentous fungi can be con-trollable, but not in levuras fungi. In some Fluconazole-added extenders (12.5mg%), ensures the solution of this problem, and even increase in sperm progressivity (8.39%) for at least a 12-hour shelf life after dilution. The validation of the experiment was done by obtaining the sow fertility rate after AI.

Keywords

Bacteria; Fungus; Boar semen; Progresivity; Fluconazole; Biosecurity of AI

Subject

LIFE SCIENCES, Biotechnology

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