Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

The New Method for Increasing the Genetic Potential in a Nucleus of Romanian Buffaloes, by Unicornual Artificial Insemination with Sexed Semen, after Stimulation with OvSynch Protocol

Version 1 : Received: 31 July 2021 / Approved: 4 August 2021 / Online: 4 August 2021 (10:02:50 CEST)

How to cite: Ciornei, S.; Drugociu, D.; Ciornei, L.M.; Petru, R. The New Method for Increasing the Genetic Potential in a Nucleus of Romanian Buffaloes, by Unicornual Artificial Insemination with Sexed Semen, after Stimulation with OvSynch Protocol. Preprints 2021, 2021080106 (doi: 10.20944/preprints202108.0106.v1). Ciornei, S.; Drugociu, D.; Ciornei, L.M.; Petru, R. The New Method for Increasing the Genetic Potential in a Nucleus of Romanian Buffaloes, by Unicornual Artificial Insemination with Sexed Semen, after Stimulation with OvSynch Protocol. Preprints 2021, 2021080106 (doi: 10.20944/preprints202108.0106.v1).

Abstract

Although artificial insemination can mean a slow and progressive improvement of animal genetics, in buffalo-cow its practical application is difficult the results are incomparably lower then cattle. The purpose of this article is to develop a new methodology, a well-known technique to make AI biotechnology more applicable to the buffalo, in order to improve the conception rate. The protocol we follow is to stimulate hormonal ovarian activity, inducing the dominant follicle, causing ovulation and scheduling the moment of sexed artificial insemination deep in the uterine horn only ipsilateral to the ovary that will ovulate. The experiment was performed on 40 primiparous buffaloes-cows in two groups for AI separated by two bulls whit 2 Millions female sperm straws. The groups was compiled after a thorough gynecological examination, and subsequently the OvSynch therapeutic protocol was started. The results were 82,5% buffaloes (33/40) had dominant follicle (DF) and inseminated, on hot/cold seasons the distribution was 75%vs90%. The conception rate was 63,6% (21/33), on hot/cold seasons 60%vs66,6%, and after calving 92.5% (20/21) female cattle were obtained. Thus, by implementing UcFTAI protocol, we state that the goal of increasing the genetic potential of CIB by becomes achievable and can be extended to a larger scale. Our Improved Protocol (UcFTAI) aims to reduce waste and maximize OvSynch hormone therapy.

Keywords

hormones; Carpathian Indigenus Buffalo; FTAI; sexed semen; OvSynch

Comments (0)

We encourage comments and feedback from a broad range of readers. See criteria for comments and our diversity statement.

Leave a public comment
Send a private comment to the author(s)
Views 0
Downloads 0
Comments 0
Metrics 0


×
Alerts
Notify me about updates to this article or when a peer-reviewed version is published.
We use cookies on our website to ensure you get the best experience.
Read more about our cookies here.