Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

A Comparison of the Capacity of Mesenchymal Stromal Cells for Cartilage Regeneration Depending on Collagen-Based Injectable Biomimetic Scaffold Type

Victor Sevastianov
* ,
Yulia Basok
ORCID logo ,
Ludmila Kirsanova
,
Alexey Grigoriev
,
Alexandra Kirillova
,
Evgeniy Nemets
,
Anastasiya Subbot
ORCID logo ,
Sergey Gautier
Version 1 : Received: 29 June 2021 / Approved: 30 June 2021 / Online: 30 June 2021 (13:11:30 CEST)

A peer-reviewed article of this Preprint also exists.

Sevastianov, V.I.; Basok, Y.B.; Kirsanova, L.A.; Grigoriev, A.M.; Kirillova, A.D.; Nemets, E.A.; Subbot, A.M.; Gautier, S.V. A Comparison of the Capacity of Mesenchymal Stromal Cells for Cartilage Regeneration Depending on Collagen-Based Injectable Biomimetic Scaffold Type. Life 2021, 11, 756. Sevastianov, V.I.; Basok, Y.B.; Kirsanova, L.A.; Grigoriev, A.M.; Kirillova, A.D.; Nemets, E.A.; Subbot, A.M.; Gautier, S.V. A Comparison of the Capacity of Mesenchymal Stromal Cells for Cartilage Regeneration Depending on Collagen-Based Injectable Biomimetic Scaffold Type. Life 2021, 11, 756.

Abstract

Mesenchymal stromal cells (MSCs) have shown a high potential for cartilage repair. Collagen-based scaffolds are used to deliver and retain cells at the site of cartilage damage. The aim of the work was a comparative analysis of the capacity of the MSCs from human adipose tissue to differentiate into chondrocytes in vitro and to stimulate the regeneration of articular cartilage in an experimental model of rabbit knee osteoarthrosis when cultured on microheterogenic collagen-based hydrogel (МCH) and the microparticles of decellularized porcine articular cartilage (DPC). The morphology of samples was evaluated using scanning electron microscopy and histological staining methods. On the surface of the DPC, the cells were distributed more uniformly than on the MCH surface. On day 28, the cells cultured on the DPC produced glycosaminoglycans more intensely compared to the MCH with the synthesis of collagen type II. However, in the experimental model of osteoarthrosis, the stimulation of the cartilage regeneration was more effective when the MSCs were administered to the MCH carrier. The present study demonstrates the way to regulate the action of the MSCs in the area of cartilage regeneration: the MCH is more conducive to stimulating cartilage repair by the MSCs, while the DPC is an inducer for a formation of a cartilage-like tissue by the MSCs in vitro.

Keywords

mesenchymal stromal cells; articular cartilage; osteoarthrosis; collagen; hydrogel; decellularization

Subject

Engineering, Automotive Engineering

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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