Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Effects of Aqueous Dispersions of C60, C70 and [email protected] Fullerenes on Genes Involved in Oxidative Stress and Anti-inflammatory Pathways

Version 1 : Received: 3 May 2021 / Approved: 7 May 2021 / Online: 7 May 2021 (09:38:38 CEST)

A peer-reviewed article of this Preprint also exists.

Proskurnina, E.V.; Mikheev, I.V.; Savinova, E.A.; Ershova, E.S.; Veiko, N.N.; Kameneva, L.V.; Dolgikh, O.A.; Rodionov, I.V.; Proskurnin, M.A.; Kostyuk, S.V. Effects of Aqueous Dispersions of C60, C70 and [email protected]82 Fullerenes on Genes Involved in Oxidative Stress and Anti-Inflammatory Pathways. Int. J. Mol. Sci. 2021, 22, 6130. Proskurnina, E.V.; Mikheev, I.V.; Savinova, E.A.; Ershova, E.S.; Veiko, N.N.; Kameneva, L.V.; Dolgikh, O.A.; Rodionov, I.V.; Proskurnin, M.A.; Kostyuk, S.V. Effects of Aqueous Dispersions of C60, C70 and [email protected] Fullerenes on Genes Involved in Oxidative Stress and Anti-Inflammatory Pathways. Int. J. Mol. Sci. 2021, 22, 6130.

Journal reference: Int. J. Mol. Sci. 2021, 22, 6130
DOI: 10.3390/ijms22116130

Abstract

Background: Fullerenes and metallofullerenes can be considered promising nanopharmaceuticals themselves and as a basis for chemical modification. As reactive oxygen species homeostasis plays a vital role in cells, the study of their effect on genes involved in oxidative stress and anti-inflammatory response is of particular importance. Methods: Human fetal lung fibroblasts were incubated with aqueous dispersions of C60, C70, and [email protected] in concentrations of 5 nM and 1.5 µM for 1, 3, 24, and 72 hours. Cell viability, intracellular ROS, NOX4, NFκB, PRAR-γ, NRF2, heme oxygenase 1, and NAD(P)H quinone dehydrogenase 1 expression have been studied. Results & conclusion: The aqueous dispersions of C60, C70, and [email protected] fullerenes are active participants in ROS homeostasis. Low and high concentrations of AFDs have similar effects. C70 was the most inert substance, C60 was the most active substance. All AFDs have both a “prooxidant” and “antioxidant” effect, but with a different balance. [email protected] was a substance with more pronounced antioxidant and anti-inflammatory properties, while C70 had more pronounced “prooxidant” properties.

Subject Areas

aqueous fullerene dispersions; pristine fullerenes; metallofullerenes; ROS homeostasis; oxidative stress; NOX4; Nrf2; PRAR-γ; heme oxygenase 1; NAD(P)H quinone dehydrogenase 1; an-ti-inflammatory pathways

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