Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Imaging the Morphological Structure of Silk Fibroin Constructs Through Fluorescence Energy Transfer and Confocal Imaging

Version 1 : Received: 2 May 2021 / Approved: 6 May 2021 / Online: 6 May 2021 (12:24:22 CEST)

A peer-reviewed article of this Preprint also exists.

Bucciarelli, A.; Quaranta, A.; Maniglio, D. Imaging the Morphological Structure of Silk Fibroin Constructs through Fluorescence Energy Transfer and Confocal Microscopy. Electron. Mater. 2021, 2, 186-197. Bucciarelli, A.; Quaranta, A.; Maniglio, D. Imaging the Morphological Structure of Silk Fibroin Constructs through Fluorescence Energy Transfer and Confocal Microscopy. Electron. Mater. 2021, 2, 186-197.

Journal reference: Electron. Mater. 2021, 2, 15
DOI: 10.3390/electronicmat2020015

Abstract

Silk fibroin is a well-known biopolymer used in several applications in which the interaction with biological tissue is required. In fact, fibroin is extremely versatile and can be shaped to form several constructs useful in tissue engineering applications. Confocal imaging is usually per-formed to test the cells behaviour on the construct and in this context the fibroin autofluorescence is regarded as a problem. In addition, the autofluorescence is not intense enough to provide useful morphological images. In fact, to control study the constructs morphology other techniques are used (i.e. SEM, Micro-CT). In this work we propose a method based on the fluorescence energy transfer (FRET) to suppress the fibroin autofluorescence moving it to higher wavelength accessible to the confocal microscopy for a direct imaging.

Subject Areas

Silk Fibroin; Fluorescence Resonance Energy Transfer (FRET); Confocal Imaging; Electrospinning

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