Preprint Review Version 1 Preserved in Portico This version is not peer-reviewed

CRISPR/Cas9 Trends and Future Scheme for Genome Engineering in Plants

Version 1 : Received: 8 March 2021 / Approved: 10 March 2021 / Online: 10 March 2021 (11:10:58 CET)

How to cite: Paudel, N.; Park, S.; Jang, Y.; Rajametov, S.N. CRISPR/Cas9 Trends and Future Scheme for Genome Engineering in Plants. Preprints 2021, 2021030277 (doi: 10.20944/preprints202103.0277.v1). Paudel, N.; Park, S.; Jang, Y.; Rajametov, S.N. CRISPR/Cas9 Trends and Future Scheme for Genome Engineering in Plants. Preprints 2021, 2021030277 (doi: 10.20944/preprints202103.0277.v1).

Abstract

The growing population meet the challenge for agricultural production. CRISPR/Cas9 technology is based on plant research for the development of the new varieties as well as disease resistance crops. In addition the deletion of significant characters makes the new alleles from the CRISPR/Cas9. Recent and reliable molecular scissor for genetic engineering. The review is focused on the various application of the CRISPR/Cas9 technology in plant enhancement of plant disease resistance, stress burden in plant, nutritional improvement, and quality of crops from the CRISPR/Cas9 system. The clustered regularly interspaced short palindromic repeat (CRISPR)-associated protein9 (cas9) is adopted from the prokaryotic type II system. CRISPR/Cas9 is simplicity and efficiency than ZENs and TALENs for the genome engineering. Due to rapid growing of the CRISPR/Cas9 system has been formulate the adaptation of many plant species. The current advancement of plants and future schemes of improve of CRISPR technology has been presented in contest of multiplex editing, knowledge on induced mutation whether the factor effect in CRISPR/Cas9 technology in plant. Remarkable perspective and challenges of CRISPR/Cas9 technology in significance of plant genetic modification.

Subject Areas

CRISPR/Cas9; Disease resistance; Future perspective; Improvement of plants; Molecular scissor; Revolution

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