RNA-sequencing Indicates High Hemocyanin Expression as a Key Strategy for Cold Adaptation in the Antarctic Amphipod Eusirus cf. giganteus Clade g3

Samuele Greco; Elisa D'Agostino; Chiara Manfrin; Anastasia Serena Gaetano; Gael Furlanis; Francesca Capanni; Gianfranco Santovito; Paolo Edomi; Piero Giulio Giulianini; Marco Gerdol

doi:10.20944/preprints202102.0226.v1

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preprints.org > biology and life sciences > anatomy and physiology > doi: 10.20944/preprints202102.0226.v1

Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

RNA-sequencing Indicates High Hemocyanin Expression as a Key Strategy for Cold Adaptation in the Antarctic Amphipod Eusirus cf. giganteus Clade g3

Samuele Greco

Elisa D'Agostino

Chiara Manfrin

Anastasia Serena Gaetano

Piero Giulio Giulianini

Marco Gerdol

Version 1 : Received: 8 February 2021 / Approved: 9 February 2021 / Online: 9 February 2021 (09:20:04 CET)

How to cite: Greco, S.; D'Agostino, E.; Manfrin, C.; Gaetano, A.S.; Furlanis, G.; Capanni, F.; Santovito, G.; Edomi, P.; Giulianini, P.G.; Gerdol, M. RNA-sequencing Indicates High Hemocyanin Expression as a Key Strategy for Cold Adaptation in the Antarctic Amphipod Eusirus cf. giganteus Clade g3. Preprints 2021, 2021020226. https://doi.org/10.20944/preprints202102.0226.v1 Greco, S.; D'Agostino, E.; Manfrin, C.; Gaetano, A.S.; Furlanis, G.; Capanni, F.; Santovito, G.; Edomi, P.; Giulianini, P.G.; Gerdol, M. RNA-sequencing Indicates High Hemocyanin Expression as a Key Strategy for Cold Adaptation in the Antarctic Amphipod Eusirus cf. giganteus Clade g3. Preprints 2021, 2021020226. https://doi.org/10.20944/preprints202102.0226.v1

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MDPI and ACS Style

Greco, S.; D'Agostino, E.; Manfrin, C.; Gaetano, A.S.; Furlanis, G.; Capanni, F.; Santovito, G.; Edomi, P.; Giulianini, P.G.; Gerdol, M. RNA-sequencing Indicates High Hemocyanin Expression as a Key Strategy for Cold Adaptation in the Antarctic Amphipod Eusirus cf. giganteus Clade g3. Preprints 2021, 2021020226. https://doi.org/10.20944/preprints202102.0226.v1

APA Style

Greco, S., D'Agostino, E., Manfrin, C., Gaetano, A.S., Furlanis, G., Capanni, F., Santovito, G., Edomi, P., Giulianini, P.G., & Gerdol, M. (2021). RNA-sequencing Indicates High Hemocyanin Expression as a Key Strategy for Cold Adaptation in the Antarctic Amphipod Eusirus cf. giganteus Clade g3. Preprints. https://doi.org/10.20944/preprints202102.0226.v1

Chicago/Turabian Style

Greco, S., Piero Giulio Giulianini and Marco Gerdol. 2021 "RNA-sequencing Indicates High Hemocyanin Expression as a Key Strategy for Cold Adaptation in the Antarctic Amphipod Eusirus cf. giganteus Clade g3" Preprints. https://doi.org/10.20944/preprints202102.0226.v1

Abstract

We here report the de novo transcriptome assembly and functional annotation of Eusirus cf. giganteus clade g3, providing the first database of expressed sequences from this giant Antarctic amphipod. RNA-sequencing, carried out on the whole-body of a single juvenile individual likely undergoing molting, revealed the dominant expression of hemocyanins. The mRNAs encoding these oxygen-binding proteins cumulatively accounted for about 40% of the total transcriptional effort, highlighting the key biological importance of high hemocyanin production in this Antarctic amphipod species. We speculate that this observation may mirror a strategy previously described in Antarctic cephalopods, which compensate the decreased ability to release oxygen to peripheral tissues at sub-zero temperatures by massively increasing total blood hemocyanin content compared with temperate species. These preliminary results will undoubtedly require confirmation through proteomic and biochemical analyses aimed at characterizing the oxygen-binding properties of E. cf. giganteus clade g3 hemocyanins, and at investigating whether other Antarctic arthropod species exploit similar adaptations to cope with the challenges posed by the extreme conditions of the polar environment.

Keywords

Antarctica; cold adaptation; hemocyanin; amphipod; transcriptome

Subject

Biology and Life Sciences, Anatomy and Physiology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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