Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

RNA-sequencing Indicates High Hemocyanin Expression as a Key Strategy for Cold Adaptation in the Antarctic Amphipod Eusirus cf. giganteus Clade g3

Version 1 : Received: 8 February 2021 / Approved: 9 February 2021 / Online: 9 February 2021 (09:20:04 CET)

How to cite: Greco, S.; D'Agostino, E.; Manfrin, C.; Gaetano, A.S.; Furlanis, G.; Capanni, F.; Santovito, G.; Edomi, P.; Giulianini, P.G.; Gerdol, M. RNA-sequencing Indicates High Hemocyanin Expression as a Key Strategy for Cold Adaptation in the Antarctic Amphipod Eusirus cf. giganteus Clade g3. Preprints 2021, 2021020226 (doi: 10.20944/preprints202102.0226.v1). Greco, S.; D'Agostino, E.; Manfrin, C.; Gaetano, A.S.; Furlanis, G.; Capanni, F.; Santovito, G.; Edomi, P.; Giulianini, P.G.; Gerdol, M. RNA-sequencing Indicates High Hemocyanin Expression as a Key Strategy for Cold Adaptation in the Antarctic Amphipod Eusirus cf. giganteus Clade g3. Preprints 2021, 2021020226 (doi: 10.20944/preprints202102.0226.v1).

Abstract

We here report the de novo transcriptome assembly and functional annotation of Eusirus cf. giganteus clade g3, providing the first database of expressed sequences from this giant Antarctic amphipod. RNA-sequencing, carried out on the whole-body of a single juvenile individual likely undergoing molting, revealed the dominant expression of hemocyanins. The mRNAs encoding these oxygen-binding proteins cumulatively accounted for about 40% of the total transcriptional effort, highlighting the key biological importance of high hemocyanin production in this Antarctic amphipod species. We speculate that this observation may mirror a strategy previously described in Antarctic cephalopods, which compensate the decreased ability to release oxygen to peripheral tissues at sub-zero temperatures by massively increasing total blood hemocyanin content compared with temperate species. These preliminary results will undoubtedly require confirmation through proteomic and biochemical analyses aimed at characterizing the oxygen-binding properties of E. cf. giganteus clade g3 hemocyanins, and at investigating whether other Antarctic arthropod species exploit similar adaptations to cope with the challenges posed by the extreme conditions of the polar environment.

Subject Areas

Antarctica; cold adaptation; hemocyanin; amphipod; transcriptome

Comments (0)

We encourage comments and feedback from a broad range of readers. See criteria for comments and our diversity statement.

Leave a public comment
Send a private comment to the author(s)
Views 0
Downloads 0
Comments 0
Metrics 0


×
Alerts
Notify me about updates to this article or when a peer-reviewed version is published.
We use cookies on our website to ensure you get the best experience.
Read more about our cookies here.