Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Self-Assembling Peptide P11-4 Presents Potential of Inducing Odontoblast-Like Cells to Biomineralization and Cell Migration

Version 1 : Received: 19 January 2021 / Approved: 20 January 2021 / Online: 20 January 2021 (12:55:47 CET)

How to cite: Araújo, I.J.D.S.; Guimarães, G.N.; Machado, R.A.; Bertassoni, L.E.; Davies, R.P.W.; Puppin-Rontani, R.M. Self-Assembling Peptide P11-4 Presents Potential of Inducing Odontoblast-Like Cells to Biomineralization and Cell Migration. Preprints 2021, 2021010398 (doi: 10.20944/preprints202101.0398.v1). Araújo, I.J.D.S.; Guimarães, G.N.; Machado, R.A.; Bertassoni, L.E.; Davies, R.P.W.; Puppin-Rontani, R.M. Self-Assembling Peptide P11-4 Presents Potential of Inducing Odontoblast-Like Cells to Biomineralization and Cell Migration. Preprints 2021, 2021010398 (doi: 10.20944/preprints202101.0398.v1).

Abstract

Self-assembling peptide P11-4 is amphiphilic and pH-triggered with demonstrated effectivity repairing early carious lesions in enamel. However, P11-4 effects on dentin biomineralization and repair remain unexplored. Thus, cytocompatibility and effectiveness of P11-4 inducing mineralization and migration of odontoblast-like cells (MDPC-23) were investigated. MDPC-23 were seeded in contact with P11-4(0.5µg/ml and 1µg/ml), Dentin Matrix Protein 1 (DMP1 0.5µg/ml and 1 µg/ml) or Calcium hydroxide (Ca(OH)2 100µg/ml) solutions. Cytotoxicity was verified using MTT (n=6/group). Mineralization was tested using Alizarin Red (n=4/group). Cell migration was assessed by light microscopy (n=2/group). MTT and Alizarin Red data were compared using Krus-kal-Wallis and Mann-Whitney (α=0.05). P11-4 (0.5µg/ml and 1µg/ml) and DMP1 (0.5µg/ml and 1µg/ml) presented the highest cytocompatibility; Ca(OH)2 presented the lowest. DMP1 1µg/ml exhibited the highest mineralization ability, with no difference to P11-4 1µg/ml. Ca(OH)2 presented lower values than DMP1 1µg/ml (p<0.05), but similar to P11-4 1µg/ml. P11-4 and DMP1 at 0.5 µg/ml showed induced less mineralization than P11-4 and DMP1 at 1µg/ml (p<0.05), with no difference to Ca(OH)2. All materials stimulated cell migration, however, lower concentrations of DMP1 and P11-4 provided better results. P11-4 is cytocompatible, induces mineralization and MDPC-23 migration like DMP1. P11-4 could be an alternative for dentin mineralization and tooth repair.

Keywords

self-assembling peptide; biomineralization; pulp-dentin complex; tooth repair; cell migration; biocompatibility

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