Preprint Communication Version 1 Preserved in Portico This version is not peer-reviewed

Microfluidic Preparation of 89Zr-Radiolabelled Proteins by Flow Photochemistry

Version 1 : Received: 18 January 2021 / Approved: 20 January 2021 / Online: 20 January 2021 (14:55:28 CET)

A peer-reviewed article of this Preprint also exists.

Earley, D.F.; Guillou, A.; van der Born, D.; Poot, A.J.; Holland, J.P. Microfluidic Preparation of 89Zr-Radiolabelled Proteins by Flow Photochemistry. Molecules 2021, 26, 764. Earley, D.F.; Guillou, A.; van der Born, D.; Poot, A.J.; Holland, J.P. Microfluidic Preparation of 89Zr-Radiolabelled Proteins by Flow Photochemistry. Molecules 2021, 26, 764.

Journal reference: Molecules 2021, 26, 764
DOI: 10.3390/molecules26030764

Abstract

89Zr-radiolabelled proteins functionalised with desferrioxamine B are a cornerstone of diagnostic positron emission tomography. In the clinical setting, 89Zr-labelled proteins are produced manually. Here, we explore the potential of using a microfluidic photochemical flow reactor to prepare 89Zr-radiolabelled proteins. The light-induced functionalisation and 89Zr-radiolabelling of human serum albumin ([89Zr]ZrDFO-PEG3-Et-azepin-HSA) was achieved by flow photochemistry with a decay-corrected radiochemical yield (RCY) of 31.2±1.3% (n = 3) and radiochemical purity >90%. In comparison, a manual batch photoreactor synthesis produced the same radiotracer in a decay-corrected RCY of 59.6±3.6% (n = 3) with an equivalent RCP >90%. The results indicate that photoradiolabelling in flow is a feasible platform for the automated production of protein-based 89Zr-radiotracers, but further refinement of the apparatus, and optimisation of the method is required before the flow process is competitive with manual reactions.

Subject Areas

Flow Chemistry; Photochemistry; Radiochemistry; Protein Conjugation.

Comments (0)

We encourage comments and feedback from a broad range of readers. See criteria for comments and our diversity statement.

Leave a public comment
Send a private comment to the author(s)
Views 0
Downloads 0
Comments 0
Metrics 0


×
Alerts
Notify me about updates to this article or when a peer-reviewed version is published.
We use cookies on our website to ensure you get the best experience.
Read more about our cookies here.