Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Hexane-Isopropanol Extraction and Quality Assessment of Omega-3 Fish Oil from Atlantic Salmon (Salmo Salar)

Version 1 : Received: 7 December 2020 / Approved: 8 December 2020 / Online: 8 December 2020 (14:55:54 CET)

How to cite: Iberahim, N.I.; Beng Choo, T.; Ahmad Sohaimi, K.S. Hexane-Isopropanol Extraction and Quality Assessment of Omega-3 Fish Oil from Atlantic Salmon (Salmo Salar). Preprints 2020, 2020120203 (doi: 10.20944/preprints202012.0203.v1). Iberahim, N.I.; Beng Choo, T.; Ahmad Sohaimi, K.S. Hexane-Isopropanol Extraction and Quality Assessment of Omega-3 Fish Oil from Atlantic Salmon (Salmo Salar). Preprints 2020, 2020120203 (doi: 10.20944/preprints202012.0203.v1).

Abstract

Atlantic salmon (Salmo Salar) is one of the fish species that rich in omega-3 fatty acids. Consumption of omega-3 fatty acids can lower down the risk of cardiovascular disease and coronary heart disease. The extraction of omega-3 fish oil from Atlantic salmon by using hexane and isopropanol was conducted in this research. Response Surface Methodology (RSM) was used to study the impact of parameters which were temperature (50-90°C), centrifuge speed (1000-3000rpm) and solvent ratio of hexane to isopropanol (0.5-1.5) to the percentage of oil yield. The results obtained shows that 15.23%± 0.61 of oil yield from Atlantic salmon under an optimum condition of 70°C, 3000 rpm and solvent ratio of hexane to isopropanol of 1.5. The acid value, peroxide value and p-anisidine value of the fish oil obtained were 7.48 mg KOH/g, 1.94 meq/kg and 5.56 respectively which were all within the acceptable limit which stipulated by Global Organization for EPA and DHA (GOED) and Food and Agricultural Organization of the United Nations (FAO). The FTIR analysis proved the presence of alkane and carboxylic acid in the fish oil. Furthermore, GC-MS analysis showed that the fish oil contains higher monounsaturated fatty acids (MUFA) contents followed by polyunsaturated fatty acids (PUFA) and 4.5525% of total omega-3 fatty acids.

Subject Areas

hexane-isopropanol extraction; omega-3 fish oil; Atlantic salmon

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