Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Application of Immobilized Cholest-4-en-3-one Δ1-Dehydrogenase from Sterolibacterium denitrificans for Dehydrogenation of Steroids

Version 1 : Received: 30 November 2020 / Approved: 1 December 2020 / Online: 1 December 2020 (08:11:00 CET)

A peer-reviewed article of this Preprint also exists.

Tataruch, M.; Wójcik, P.; Wojtkiewicz, A.M.; Zaczyk, K.; Szymańska, K.; Szaleniec, M. Application of Immobilized Cholest-4-En-3-One Δ1-Dehydrogenase from Sterolibacterium Denitrificans for Dehydrogenation of Steroids. Catalysts 2020, 10, 1460. Tataruch, M.; Wójcik, P.; Wojtkiewicz, A.M.; Zaczyk, K.; Szymańska, K.; Szaleniec, M. Application of Immobilized Cholest-4-En-3-One Δ1-Dehydrogenase from Sterolibacterium Denitrificans for Dehydrogenation of Steroids. Catalysts 2020, 10, 1460.

Journal reference: Catalysts 2020, 10, 1460
DOI: 10.3390/catal10121460

Abstract

Cholest-4-en-3-one Δ1-dehydrogenase (AcmB) from Sterolibacterium denitrificans is successfully immobilized on 3-aminopropyltrimethoysilane functionalized MCF and SBA-15 silica supports using adsorption or covalently with glutaraldehyde or divinyl sulfone linkers. The best catalyst, AcmB on MCF linked covalently with glutaraldehyde, retains the specific activity of the homogenous enzyme while exhibiting a substantial increase of the operational stability. The immobilized enzyme was used continuously in the fed-batch reactor for 27 days, catalyzing 1,2-dehydrogenation of androst-4-en-3-one to androst-1,4-dien-3-one with a final yield of 29.9 mM (8.56 g/L) and 99% conversion. The possibility of reuse of the immobilized catalyst was also demonstrated and resulted with a doubling of the product amount compared to that in the reference homogenous reactor. Finally, it was shown that molecular oxygen from the air can efficiently be used as an electron acceptor either reoxidizing directly the enzyme or the reduced DCPIPH2. Keywords: 3-ketosteroid D1-dehydrogenase; KSTD; KSDH; AcmB; 1,2-dehydrogenation; cholest-4-en-3-one Δ1-dehydrogenase; enzyme immobilization, FAD-dependent enzymes; enzyme immobilization;

Subject Areas

3-ketosteroid Δ1-dehydrogenase; KSTD; KSDH; AcmB; 1,2-dehydrogenation; cholest-4-en-3-one Δ1-dehydrogenase; enzyme immobilization; FAD-dependent enzymes; enzyme immobilization

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