Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Huanglian Decoction Suppresses the Growth of Hepatocellular Carcinoma Cells by Reducing CCNB1 Expression

Version 1 : Received: 26 November 2020 / Approved: 27 November 2020 / Online: 27 November 2020 (13:14:24 CET)

How to cite: Li, M.; Shang, H.; Wang, T.; Yang, S.; li, L. Huanglian Decoction Suppresses the Growth of Hepatocellular Carcinoma Cells by Reducing CCNB1 Expression. Preprints 2020, 2020110691 (doi: 10.20944/preprints202011.0691.v1). Li, M.; Shang, H.; Wang, T.; Yang, S.; li, L. Huanglian Decoction Suppresses the Growth of Hepatocellular Carcinoma Cells by Reducing CCNB1 Expression. Preprints 2020, 2020110691 (doi: 10.20944/preprints202011.0691.v1).

Abstract

Background: Hepatocellular carcinoma (HCC) is one of the most prevalent cancers in human populations worldwide. Conversely, Huanglian Decoction is one of the most important Chinese medicine formulas, with the potential to treat cancer. Methods: To identify differentially expressed genes (DEG), we herein downloaded gene expression profile data from the TCGA (TCGA-LIHC) and GEO (GSE45436) databases. We obtained phytochemicals of the four constituent herbs of Huanglian Decoction from the traditional Chinese medicine systems pharmacology database and analysis platform (TCMSP). We also established a regulatory network of DEG and their drug target genes and subsequently analyzed key genes using bioinformatic approaches. Furthermore, we explored the effect of Huanglian Decoction by conducting in vitro experiments so as to verify the prediction. In particular, the CCNB1 gene was knockdown to verify the primary target of this Decoction. Results: Based on the results of network pharmacological analysis, we revealed that there are 5 bioactive compounds in Huanglian Decoction acting on HCC. In addition, our findings confirmed that CCNB1 was the primary key gene, which can be highly expressed in tumors and was significantly associated with a worse prognosis (P = 0.002) according to PPI network analysis of the target genes of these five compounds, as well as expression and prognosis analyses in tumors. We also noted that CCNB1 can be used as an independent prognostic indicator of HCC (P < 0.01). Moreover, in vitro experimental results demonstrate that Huanglian Decoction can significantly inhibit the growth, migration, and invasion of HCC cells. Finally, further analysis showed that this Decoction may inhibit the growth of HCC cells by down-regulating the expression level of CCNB1.

Subject Areas

Huanglian Decoction (Coptidis Rhizoma, Zingiberis Rhizoma, Folium Artemisiae Argyi, Mume Fructus); Hepatocellular carcinoma; TCGA; GEO

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