Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Association Mapping for Drought Tolerance and Yield-Related Traits in Cowpea Accessions

Version 1 : Received: 30 September 2020 / Approved: 1 October 2020 / Online: 1 October 2020 (08:40:47 CEST)

How to cite: Nkomo, G.V.; Sedibe, M.; Mofokeng, M.A.; Pierneef, R. Association Mapping for Drought Tolerance and Yield-Related Traits in Cowpea Accessions. Preprints 2020, 2020100001 (doi: 10.20944/preprints202010.0001.v1). Nkomo, G.V.; Sedibe, M.; Mofokeng, M.A.; Pierneef, R. Association Mapping for Drought Tolerance and Yield-Related Traits in Cowpea Accessions. Preprints 2020, 2020100001 (doi: 10.20944/preprints202010.0001.v1).

Abstract

The objective of this study were to conduct association mapping for drought tolerance at the seedling stage and yield-related traits. 60 cowpea accessions were used in the study. Single-nucleotide polymorphisms (SNPs) discovered through genotyping by sequencing (GBS) were used for genotyping. Association mapping was conducted using single-marker regression (SMR) in Q Gene, and general linear model (GLM) and mixed linear model (MLM) built in TASSEL. The population of the cowpea accessions were analysed using STRUCTURE 2.3.4 and the peak of delta K in the greenhouse showed seven population types, whereas the peak of delta K in the glasshouse indicated the presence of six population types. One SNP marker, 14083649|F|0-9 was associated with NP with a p value <0.001. Fifty SNP markers were associated with PWT at p <0.001. Four SNP markers, 14074781|F|0-16, 100047392|F|0-36, 14083801|F|0-28 and 100051488|F|0-49 were associated with AVSPD at p <0.001. SNP markers, 14074781|F|0-16, 14083801|F|0-28 and 100051488|F|0-49 were associated with PL at P <0.001. Five SNP markers, 100047392|F|0-36, 14083801|F|0-28, 100072738|F|0-34, 14076881|F|0-49 and 14076881|F|0-49 were associated with PWDTH at p <0.001. The 65 SNP markers identified can be used in cowpea molecular breeding to select for AVSPD, NP, PL, PWDTH, PWT, and RR through marker assisted selection (MAS).

Subject Areas

Association mapping; chromosomes; drought tolerance; markers, structure; traits

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