Version 1
: Received: 30 September 2020 / Approved: 1 October 2020 / Online: 1 October 2020 (08:40:47 CEST)
How to cite:
Nkomo, G.V.; Sedibe, M.; Mofokeng, M.A.; Pierneef, R. Association Mapping for Drought Tolerance and Yield-Related Traits in Cowpea Accessions. Preprints2020, 2020100001. https://doi.org/10.20944/preprints202010.0001.v1
Nkomo, G.V.; Sedibe, M.; Mofokeng, M.A.; Pierneef, R. Association Mapping for Drought Tolerance and Yield-Related Traits in Cowpea Accessions. Preprints 2020, 2020100001. https://doi.org/10.20944/preprints202010.0001.v1
Nkomo, G.V.; Sedibe, M.; Mofokeng, M.A.; Pierneef, R. Association Mapping for Drought Tolerance and Yield-Related Traits in Cowpea Accessions. Preprints2020, 2020100001. https://doi.org/10.20944/preprints202010.0001.v1
APA Style
Nkomo, G.V., Sedibe, M., Mofokeng, M.A., & Pierneef, R. (2020). Association Mapping for Drought Tolerance and Yield-Related Traits in Cowpea Accessions. Preprints. https://doi.org/10.20944/preprints202010.0001.v1
Chicago/Turabian Style
Nkomo, G.V., Maletsema Alina Mofokeng and Rian Pierneef. 2020 "Association Mapping for Drought Tolerance and Yield-Related Traits in Cowpea Accessions" Preprints. https://doi.org/10.20944/preprints202010.0001.v1
Abstract
The objective of this study were to conduct association mapping for drought tolerance at the seedling stage and yield-related traits. 60 cowpea accessions were used in the study. Single-nucleotide polymorphisms (SNPs) discovered through genotyping by sequencing (GBS) were used for genotyping. Association mapping was conducted using single-marker regression (SMR) in Q Gene, and general linear model (GLM) and mixed linear model (MLM) built in TASSEL. The population of the cowpea accessions were analysed using STRUCTURE 2.3.4 and the peak of delta K in the greenhouse showed seven population types, whereas the peak of delta K in the glasshouse indicated the presence of six population types. One SNP marker, 14083649|F|0-9 was associated with NP with a p value <0.001. Fifty SNP markers were associated with PWT at p <0.001. Four SNP markers, 14074781|F|0-16, 100047392|F|0-36, 14083801|F|0-28 and 100051488|F|0-49 were associated with AVSPD at p <0.001. SNP markers, 14074781|F|0-16, 14083801|F|0-28 and 100051488|F|0-49 were associated with PL at P <0.001. Five SNP markers, 100047392|F|0-36, 14083801|F|0-28, 100072738|F|0-34, 14076881|F|0-49 and 14076881|F|0-49 were associated with PWDTH at p <0.001. The 65 SNP markers identified can be used in cowpea molecular breeding to select for AVSPD, NP, PL, PWDTH, PWT, and RR through marker assisted selection (MAS).
Keywords
Association mapping; chromosomes; drought tolerance; markers, structure; traits
Subject
Biology and Life Sciences, Agricultural Science and Agronomy
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
