Version 1
: Received: 30 July 2020 / Approved: 31 July 2020 / Online: 31 July 2020 (09:31:18 CEST)
How to cite:
Morehouse, Z.P.; Proctor, C.M.; Ryan, G.L.; Nash, R.J. Speeding Up COVID-19 Detection Using Shaker-Mill Homogenization and a Direct-to-PCR Workflow. Preprints2020, 2020070740. https://doi.org/10.20944/preprints202007.0740.v1
Morehouse, Z.P.; Proctor, C.M.; Ryan, G.L.; Nash, R.J. Speeding Up COVID-19 Detection Using Shaker-Mill Homogenization and a Direct-to-PCR Workflow. Preprints 2020, 2020070740. https://doi.org/10.20944/preprints202007.0740.v1
Morehouse, Z.P.; Proctor, C.M.; Ryan, G.L.; Nash, R.J. Speeding Up COVID-19 Detection Using Shaker-Mill Homogenization and a Direct-to-PCR Workflow. Preprints2020, 2020070740. https://doi.org/10.20944/preprints202007.0740.v1
APA Style
Morehouse, Z.P., Proctor, C.M., Ryan, G.L., & Nash, R.J. (2020). Speeding Up COVID-19 Detection Using Shaker-Mill Homogenization and a Direct-to-PCR Workflow. Preprints. https://doi.org/10.20944/preprints202007.0740.v1
Chicago/Turabian Style
Morehouse, Z.P., Gabriella L Ryan and Rodney J Nash. 2020 "Speeding Up COVID-19 Detection Using Shaker-Mill Homogenization and a Direct-to-PCR Workflow" Preprints. https://doi.org/10.20944/preprints202007.0740.v1
Abstract
Accurate and timely testing has become an essential measure in combatting the COVID-19 global pandemic. Currently, polymerase chain reaction (PCR) based assays are the most relied on methods for SARS-CoV-2 detection. This traditional workflow involves a viral RNA extraction from the viral transport media storing nasopharyngeal swabs collected from patients, followed by PCR based detection. While accurate, this methodology is time consuming and resource heavy, causing for delays in receiving results or limited access to testing. Herein, we demonstrate a validated method for SARS-CoV-2 detection from viral transport media using a two-step, direct-to-PCR workflow revolving around shaker-mill homogenization. This method completely bypasses the extraction steps of the traditional workflow, replacing it with 30 seconds of mechanical disruption sufficient to allow for COVID-19 detection with a 96.43% sensitivity and 100% specificity when compared to traditional extraction to PCR based methods.
Medicine and Pharmacology, Epidemiology and Infectious Diseases
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
