Preprint Article Version 1 This version is not peer-reviewed

Animal Food Allergens Share the Formation of IgE-binding Amyloids

Version 1 : Received: 14 July 2020 / Approved: 16 July 2020 / Online: 16 July 2020 (13:31:23 CEST)

How to cite: Pérez-Tavarez, R.; Castellanos, M.; Loli-Ausejo, D.; Pedrosa, M.; Hurtado, J.L.; Rodriguez-Pérez, R.; Gasset, M. Animal Food Allergens Share the Formation of IgE-binding Amyloids. Preprints 2020, 2020070359 (doi: 10.20944/preprints202007.0359.v1). Pérez-Tavarez, R.; Castellanos, M.; Loli-Ausejo, D.; Pedrosa, M.; Hurtado, J.L.; Rodriguez-Pérez, R.; Gasset, M. Animal Food Allergens Share the Formation of IgE-binding Amyloids. Preprints 2020, 2020070359 (doi: 10.20944/preprints202007.0359.v1).

Abstract

Several animal food allergens assembly into amyloids under gastric-like environments. These aggregated structures provide Gad m 1 with an enhanced IgE interaction due to the amyloid assembly of the epitope regions. However, whether these properties are unique of Gad m 1 or common to other food allergens has not yet been addressed. Using Bos d 5, Bos d 12 and Gal d 2 as food allergen models and Gad m 1 as control, aggregation reactions and the sera of milk, egg and fish allergic patients we have analyzed the IgE interaction of the distinct amyloids. We found that amyloids formed by Bos d 12 and Gal d 2 full-length and truncated chains are recognized by the IgEs of milk and egg allergic patient sera. As with Gad m 1, in most cases amyloid recognition is higher than that of the precursor structure. Bos d 5 was not recognized under any fold by the IgE of the sera studied. These results support that formation of IgE-binding amyloids might be a common feature to animal food allergen.

Subject Areas

food allergens; egg allergy; milk allergy; fish allergy; amyloids; IgE-binding

Comments (0)

We encourage comments and feedback from a broad range of readers. See criteria for comments and our diversity statement.

Leave a public comment
Send a private comment to the author(s)
Views 0
Downloads 0
Comments 0
Metrics 0


×
Alerts
Notify me about updates to this article or when a peer-reviewed version is published.