Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Engineered Durum Wheat Germplasm with Multiple Alien Introgressions: Agronomic and Quality Performance

Version 1 : Received: 26 February 2020 / Approved: 27 February 2020 / Online: 27 February 2020 (11:29:25 CET)

A peer-reviewed article of this Preprint also exists.

Kuzmanović, L.; Rossini, F.; Ruggeri, R.; Pagnotta, M.A.; Ceoloni, C. Engineered Durum Wheat Germplasm with Multiple Alien Introgressions: Agronomic and Quality Performance. Agronomy 2020, 10, 486. Kuzmanović, L.; Rossini, F.; Ruggeri, R.; Pagnotta, M.A.; Ceoloni, C. Engineered Durum Wheat Germplasm with Multiple Alien Introgressions: Agronomic and Quality Performance. Agronomy 2020, 10, 486.

Journal reference: Agronomy 2020, 10, 486
DOI: 10.3390/agronomy10040486

Abstract

If genetic gains in wheat yield are to be achieved in today’s breeding, increasing genetic variability of cultivated genotypes is an essential requisite to meet. To this aim, alien gene transfer through chromosome engineering (CE) is a validated and sound strategy. Attempts to incorporate more than one alien segment into cultivated wheat have been rare, particularly for tetraploid durum wheat. Here we present the agronomic and quality performance of the first successful CE-mediated multiple introgression into the latter species. By assembling into 7AL, 3BS and 1AS arms of a single genotype homoeologous segments of Thinopyrum ponticum 7el1L, Aegilops longissima 3SlS, and Triticum aestivum 1DS arms, respectively, we have stacked several valuable alien genes, comprising Lr19+Sr25+Yp (leaf and stem rust resistance and a gene increasing semolina yellowness), Pm13 (powdery mildew resistance) and Gli-D1/Glu-D3 (genes affecting gluten properties), respectively. Advanced progenies of single, double and triple recombinants were field-tested across three years in a typical durum wheat growing area of Central Italy. The results showed that not only all recombinants had normal phenotype and fertility, but also that one of the triple recombinants had the highest yield through all seasons compared with all other recombinants and control cultivars. Moreover, the multiple introgressions enhanced quality traits, including gluten characteristics and semolina yellow index. Presence of effective disease resistance genes confers additional breeding value to the novel and functional CE products, which can greatly contribute to crop security and safety.

Subject Areas

chromosome engineering; wheat breeding; Aegilops longissima; Thinopyrum ponticum; gluten quality; yield; leaf rust; stem rust; powdery mildew

Comments (0)

We encourage comments and feedback from a broad range of readers. See criteria for comments and our diversity statement.

Leave a public comment
Send a private comment to the author(s)
Views 0
Downloads 0
Comments 0
Metrics 0


×
Alerts
Notify me about updates to this article or when a peer-reviewed version is published.
We use cookies on our website to ensure you get the best experience.
Read more about our cookies here.