Working Paper Article Version 1 This version is not peer-reviewed

Overexpression of GmRIQ2-like (Glyma.04G174400) Enhances the Tolerance of Strong Light Stress and Reduces Photoinhibition in (Glycine max L. Merr.)

Version 1 : Received: 7 February 2020 / Approved: 10 February 2020 / Online: 10 February 2020 (09:47:05 CET)

A peer-reviewed article of this Preprint also exists.

Deng, J.; Li, D.; Yin, H.; Ma, L.; Zhang, J.; Zhang, B. Overexpression of GmRIQ2-Like (Glyma.04G174400) Enhances the Tolerance of Strong Light Stress and Reduces Photoinhibition in Soybean (Glycine max (L.) Merr.). Agriculture 2020, 10, 157. Deng, J.; Li, D.; Yin, H.; Ma, L.; Zhang, J.; Zhang, B. Overexpression of GmRIQ2-Like (Glyma.04G174400) Enhances the Tolerance of Strong Light Stress and Reduces Photoinhibition in Soybean (Glycine max (L.) Merr.). Agriculture 2020, 10, 157.

Journal reference: Agriculture 2020, 10, 157
DOI: 10.3390/agriculture10050157

Abstract

Soybean (Glycine max L.) is an important crop that serves as a source of edible oil and protein. However, little is known about its molecular mechanism of adaptation to extreme environmental conditions. Based on the Arabidopsis thaliana sequence database and Phytozome, a soybean gene that was highly homogenous with the reduced induction of the non-photochemical quenching2 (AtRIQ2) gene, GmRIQ2-like (accession NO.: Glyma.04G174400), was identified in this study. The gene structure analysis revealed that GmRIQ2-like encoded a transmembrane protein. Elements of the promoter analysis indicated that GmRIQ2-like participated in the photosynthesis and abiotic stress pathways. The subcellular localization results revealed that the protein encoded by GmRIQ2-like was located in chloroplasts. The quantitative real-time (qRT)-PCR results revealed that GmRIQ2-like-overexpression (OE) and -knock-out (KO) transgenic soybean seedlings were cultivated successfully. The relative chlorophyll (Chl) and zeaxanthin contents and Chl fluorescence kinetic parameters demonstrated that GmRIQ2-like dissipated excess light energy by enhancing the non-photochemical quenching (NPQ) and reduced plant photoinhibition. These results suggested that GmRIQ2-like was induced in response to strong light and depressed Chl production involved in soybean stress tolerance. These findings indicate that the transgenic seedlings of GmRIQ2-like could be used to enhance strong light stress tolerance and protect soybean plants from photoinhibition damage. This study will serve as a reference for studying crop photoprotection regulation mechanisms and benefits the research and development of new cultivars.

Subject Areas

soybean (Glycine max L.); NPQ; photoinhibition; bioinformatics; subcellular localization; qRT-PCR

Comments (0)

We encourage comments and feedback from a broad range of readers. See criteria for comments and our diversity statement.

Leave a public comment
Send a private comment to the author(s)
Views 0
Downloads 0
Comments 0
Metrics 0


×
Alerts
Notify me about updates to this article or when a peer-reviewed version is published.
We use cookies on our website to ensure you get the best experience.
Read more about our cookies here.