Preprint Brief Report Version 1 Preserved in Portico This version is not peer-reviewed

Summary: Chemical Modulation of Circadian Rhythms and Assessment of Cellular Behavior via Indirubin and Derivatives

Version 1 : Received: 23 January 2020 / Approved: 25 January 2020 / Online: 25 January 2020 (00:33:40 CET)

A peer-reviewed article of this Preprint also exists.

Journal reference: Methods in Enzymology 2020, 639, 115-140
DOI: 10.1016/bs.mie.2020.04.011


We present a summary of our protocol for employment of small-molecule circadian rhythm modulators. The abilities of compounds to affect oscillations have received significant attention. We outline assessments of circadian changes using indirubin-3’-oxime (IO), 5-iodo-indirubin-3’-oxime (5I-IO), and 5-sulfonic acid-indirubin-3’-oxime (5SA-IO), analyzing effects on Bmal1 and Per2 oscillations and oncogenic features.


circadian rhythms; small molecules; luciferase reporters; period estimations



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