Lin, H.-H.; Robertson, K.L.; Lellupitiyage Don, S.S.; Taylor, S.R.; Farkas, M.E. Chemical Modulation of Circadian Rhythms and Assessment of Cellular Behavior via Indirubin and Derivatives. Methods in Enzymology 2020, 115–140, doi:10.1016/bs.mie.2020.04.011.
Lin, H.-H.; Robertson, K.L.; Lellupitiyage Don, S.S.; Taylor, S.R.; Farkas, M.E. Chemical Modulation of Circadian Rhythms and Assessment of Cellular Behavior via Indirubin and Derivatives. Methods in Enzymology 2020, 115–140, doi:10.1016/bs.mie.2020.04.011.
Lin, H.-H.; Robertson, K.L.; Lellupitiyage Don, S.S.; Taylor, S.R.; Farkas, M.E. Chemical Modulation of Circadian Rhythms and Assessment of Cellular Behavior via Indirubin and Derivatives. Methods in Enzymology 2020, 115–140, doi:10.1016/bs.mie.2020.04.011.
Lin, H.-H.; Robertson, K.L.; Lellupitiyage Don, S.S.; Taylor, S.R.; Farkas, M.E. Chemical Modulation of Circadian Rhythms and Assessment of Cellular Behavior via Indirubin and Derivatives. Methods in Enzymology 2020, 115–140, doi:10.1016/bs.mie.2020.04.011.
Abstract
We present a summary of our protocol for employment of small-molecule circadian rhythm modulators. The abilities of compounds to affect oscillations have received significant attention. We outline assessments of circadian changes using indirubin-3’-oxime (IO), 5-iodo-indirubin-3’-oxime (5I-IO), and 5-sulfonic acid-indirubin-3’-oxime (5SA-IO), analyzing effects on Bmal1 and Per2 oscillations and oncogenic features.
Keywords
circadian rhythms; small molecules; luciferase reporters; period estimations
Subject
Biology and Life Sciences, Biochemistry and Molecular Biology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
