Preprint Article Version 1 This version is not peer-reviewed

The Role of Purified α-amylase from Soil Bacteria Versus Crude Cell Supernatant from Medium against Clinical Biofilm-forming Bacteria

Version 1 : Received: 24 December 2019 / Approved: 25 December 2019 / Online: 25 December 2019 (09:48:15 CET)

How to cite: Elamary, R.; Albarakaty, F.; Salem, W. The Role of Purified α-amylase from Soil Bacteria Versus Crude Cell Supernatant from Medium against Clinical Biofilm-forming Bacteria. Preprints 2019, 2019120344 (doi: 10.20944/preprints201912.0344.v1). Elamary, R.; Albarakaty, F.; Salem, W. The Role of Purified α-amylase from Soil Bacteria Versus Crude Cell Supernatant from Medium against Clinical Biofilm-forming Bacteria. Preprints 2019, 2019120344 (doi: 10.20944/preprints201912.0344.v1).

Abstract

Bacterial biofilms have become a significant and growing threat to human life, nature, and environmental health. The aim of this study is to isolate amylase-producing bacteria from the terrestrial environment (soil) for investigating their general inhibition of some pathogenic human bacterial biofilm. A total of 75 amylase producing isolates were obtained by serial dilution and streaking method. Amylotic activity of these isolates was screened by a starch agar plate method. Isolates were characterized by morphological and biochemical methods. The isolated Bacillus species were B. megaterium (26.7%), B. subtilis (16%), B. cereus (13.3%), B. thuringiesis (10.7%), B. lentus (10.7%), B. mycoides (5.3%), B. alvei (5.3%), B. polymyxa (4%), B. circulans (4%) and Micrococcus roseus (4%). The optimum conditions for amylase production were monitored. Antagonistic activity of these isolates and purified amylase against multidrug-resistant pathogenic human bacteria by agar disk diffusion method. The sensitivity level of some standard antibiotics served as control. Interestingly, it was found that all isolates exhibited great antagonistic activity against the target pathogens. The greatest activity recoded by B. alevi (48 mm) against Staphylococcus aureus (MRSA) and the lowest activity recorded by B. polymyxa (12 mm) against E. coli while low inhibition recorded for amylase. Antibiofilm efficacy of isolates supernatant and purified enzyme also monitored by spectrophotometric methods. The results revealed highly significant inhibition with percentages of 93.6 and 78.8% respectively. So, they represent a good tool for biofilm control in clinical and environmental applications.

Subject Areas

Amylase; Bacillus; Soil bacteria; Antibiofilm; Pathogenic bacteria

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