Working Paper Article Version 2 This version is not peer-reviewed

Protective Effects of Icariin on Nicotine-induced Reproductive Toxicity in Male Mouse

Version 1 : Received: 3 October 2019 / Approved: 8 October 2019 / Online: 8 October 2019 (10:30:43 CEST)
Version 2 : Received: 9 October 2019 / Approved: 14 October 2019 / Online: 14 October 2019 (09:58:40 CEST)

How to cite: Ni, G.; Zhang, X.; Afedo, S.Y.; Rui, R. Protective Effects of Icariin on Nicotine-induced Reproductive Toxicity in Male Mouse. Preprints 2019, 2019100082 Ni, G.; Zhang, X.; Afedo, S.Y.; Rui, R. Protective Effects of Icariin on Nicotine-induced Reproductive Toxicity in Male Mouse. Preprints 2019, 2019100082

Abstract

Nicotine is a pharmacologically active component of tobacco which adversely affects the male reproductive system and fertility and Icariin (ICA) is the main active ingredient of Epimedium herba which has been used to treat several male reproductive problems. This study was aimed at investigating the protective or ameliorative effect of ICA against reproductive toxicity induced by intraperitoneal injection of nicotine in mice. Forty male mice were randomly divided into 4 groups: control, nicotine (0.75 mg/kg intraperitoneally), icariin (ICA, 75 mg/kg), and icariin plus nicotine (ICA + nicotine) group. After 35 days of treatment, the mice were weighed, sacrificed, and their reproductive organs were collected and examined for further studies. In the nicotine-treated group, epididymal sperm density and serum testosterone concentrations significantly decreased relative to the control group. Nicotine also caused oxidative damage as shown by significant reduction in the activities of antioxidant enzymes and an elevation in Malondialdehyde (MDA) levels. Icariin on the other hand, improved the reduction in sperm characteristics, hormone levels, and activities of antioxidant enzymes alterations observed in the nicotine treated mice. These findings indicate that the nicotine-induced reproductive toxicity and oxidative damages on male reproductive tissues can be effectively attenuated by icariin.

Subject Areas

icariin; nicotine; sperm density; testosterone; antioxidant enzyme; male mice

Comments (1)

Comment 1
Received: 14 October 2019
Commenter: Seth Yaw Afedo
Commenter's Conflict of Interests: Author
Comment: 1. In the abstract, line 19; reproductive parameters has changed to reproductive organs.
2. In table 1, the superscript for Nicotine under Testicular weight is “a” thus should appear as 0.279±0.019a
3. Also in table 1, the superscript for Nicotine under Testicular index is “a” thus should appear as 5.22±0.15a
4. Again in table 1, the superscript for ICA+Nicotine under Testicular index is “ab” thus should appear as 5.56±0.45ab
5. In line 89,we wish to have: #: P < 0.05 vs. control mice; *: P < 0.05 vs. nicotine treated rats. 6. In table 2, the superscript for ICA+Nicotine under MDA(nmol/g protein) is “a” thus should appear as 3.82±0.57a
7. In line 105 – 106, the sentence should read as Furthermore, the concentration of MDA in icariin group were significantly lower than that of the control group (P>0.05; Table 2). 8. In line 114, (Figure 3A).
9. In line 116, compared
10. In line 118, compared
11. In line, 120, (Figure 3B).
12. In line 131, 0.75 mg/kg, please i.p. has been deleted
13. In line 132-133, the sentence should read as “The results of this study showed that nicotine administration had no significant effect on body weight, absolute, and relative testicular weight.”
14. In line 152, we wish to have “…reversed due to the co-treatment with ICA. This study suggests a possible mechanism of ICA in…”
15. In line183-186, the beginning of point 4.2. Animals and experimental design should be: All procedures and protocols involving animals were in accordance with the Animal Ethics Procedure and Guidelines of the People’s Republic of China and the Guide for the Care and Use of Laboratory Animals. All animal procedures were also approved by the Institutional Animal Care and Use Committee (IACUC) of Nanjing Agricultural University with Permit No. 2018CB114306.
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