Preprint Article Version 1 This version is not peer-reviewed

Hypolipidemic Effects and Mechanisms of Val-Phe-Val-Arg-Asn in C57BL/6J Mice and 3T3-L1 Cell Models

Version 1 : Received: 26 September 2019 / Approved: 27 September 2019 / Online: 27 September 2019 (10:29:29 CEST)

How to cite: W, S.; Hui, H.; Ruge, C.; Tao, H. Hypolipidemic Effects and Mechanisms of Val-Phe-Val-Arg-Asn in C57BL/6J Mice and 3T3-L1 Cell Models. Preprints 2019, 2019090309 (doi: 10.20944/preprints201909.0309.v1). W, S.; Hui, H.; Ruge, C.; Tao, H. Hypolipidemic Effects and Mechanisms of Val-Phe-Val-Arg-Asn in C57BL/6J Mice and 3T3-L1 Cell Models. Preprints 2019, 2019090309 (doi: 10.20944/preprints201909.0309.v1).

Abstract

Val-Phe-Val-Arg-Asn (VFVRN) has been identified and screened from lipid-lowering chickpea peptides (ChPs) by using a pharmacokinetic model in our previous experiment. The present study was conducted to investigate its effects and mechanisms on lipid metabolism. A high-fat diet C57BL/6J mice model and 3T3-L1 preadipocyte cell model were used. VFVRN was found to significantly decrease the levels of some blood lipids. The expressions of LDL receptor (LDLR), peroxisome proliferator-activated receptors (PPAR)α, liver X receptor (LXR)α, cholesterol 7α-hydroxylase (CYP7A1) and AMP-activated protein kinase (p-AMPK) in liver were up-regulated by VFVRN treatment. The expressions of 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMGR), fatty acid synthetase (FAS), 1-aminocyclopropane-1-carboxylate synthetase (ACC), sterol regulatory element-binding protein (SREBP)-1c and SREBP-2 in liver were significantly (P<0.05) down-regulated. Additionally, the expressions of PPARα and PPARγ in adipose tissues were up-regulated by VFVRN significantly (P<0.05). VFVRN might also contribute to transintestinal cholesterol efflux (TICE) by up-regulating the expressions of LXRα and ATP binding cassette G5/8 transporters (ABGC5/8). Moreover, VFVRN promoted 3T3-L1 preadipocyte apoptosis by up-regulating the expressions of BaX, cleaved Caspase-3 and down-regulating Bcl-2. VFVRN had potent effects in reversing metabolic disorders of blood and liver in a high-fat diet mice model, as well as to promote the apoptosis of 3T3-L1 preadipocytes.

Subject Areas

val-phe-val-arg-asn (vfvrn); hypolipidemic effects; transintestinal cholesterol efflux (tice); 3t3-l1 preadipocyte; apoptosis

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