Abril, D.J.; Bustos Moya, I.G.; Marquez-Ortiz, R.A.; Josa Montero, D.F.; Corredor Rozo, Z.L.; Torres Molina, I.; Vanegas Gómez, N.; Escobar-Perez, J. First Report and Comparative Genomics Analysis of a blaOXA-244–Harbouring Escherichia coli Isolate Recovered in American Continent. Preprints2019, 2019090150. https://doi.org/10.20944/preprints201909.0150.v1
APA Style
Abril, D.J., Bustos Moya, I.G., Marquez-Ortiz, R.A., Josa Montero, D.F., Corredor Rozo, Z.L., Torres Molina, I., Vanegas Gómez, N., & Escobar-Perez, J. (2019). First Report and Comparative Genomics Analysis of a <em>bla</em><sub>OXA-244</sub>–Harbouring <em>Escherichia coli </em>Isolate Recovered in American Continent. Preprints. https://doi.org/10.20944/preprints201909.0150.v1
Chicago/Turabian Style
Abril, D.J., Natasha Vanegas Gómez and Javier Escobar-Perez. 2019 "First Report and Comparative Genomics Analysis of a <em>bla</em><sub>OXA-244</sub>–Harbouring <em>Escherichia coli </em>Isolate Recovered in American Continent" Preprints. https://doi.org/10.20944/preprints201909.0150.v1
Abstract
The carbapenemase OXA-244 is a derivate of OXA-48, and its detection is very difficult in laboratories. Here we report the identification and genomic analysis of an Escherichia coli isolate (28Eco12) harbouring the blaOXA-244 gene identified in Colombia, South America. The 28Eco12 isolate was identified during a retrospective study and it was recovered from a patient treated in Colombia. The complete nucleotide sequence was established using the PacBio platform. A comparative genomics analysis with other blaOXA-244–harbouring Escherichia coli strains was performed. The 28Eco12 isolate belonged to sequence type (ST) 38 and its genome was composed of two molecules, a chromosome of 5,343,367 bp and a plasmid of 92,027 bp, which belonged to the incompatibility group IncY and did not harbour resistance genes. The blaOXA-244 gene was chromosomally-encoded and mobilized by an ISR1-related Tn6237 composite transposon. Notably, this transposon was inserted and located within a new genomic island. For our knowledge this is the first report of a blaOXA-244–harbouring Escherichia coli isolate in American continent.Our results suggest that the introduction of the OXA-244-producing E. coli isolate was through clonal expansion of the ST38 pandemic clone. Other isolates producing OXA-244 could be circulating silently on the American continent.
Biology and Life Sciences, Immunology and Microbiology
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