Preprint Review Version 1 This version is not peer-reviewed

In Vivo Methods to Study Protein-Protein Interactions as Key Players in Mycobacterium tuberculosis Virulence

Version 1 : Received: 28 August 2019 / Approved: 29 August 2019 / Online: 29 August 2019 (08:46:44 CEST)

A peer-reviewed article of this Preprint also exists.

Veyron-Churlet, R.; Locht, C. In Vivo Methods to Study Protein–Protein Interactions as Key Players in Mycobacterium Tuberculosis Virulence. Pathogens 2019, 8, 173. Veyron-Churlet, R.; Locht, C. In Vivo Methods to Study Protein–Protein Interactions as Key Players in Mycobacterium Tuberculosis Virulence. Pathogens 2019, 8, 173.

Journal reference: Pathogens 2019, 8, 173
DOI: 10.3390/pathogens8040173

Abstract

Studies on Protein-Protein interactions (PPI) can be helpful for the annotation of unknown protein function and for the understanding of cellular processes, such as specific virulence mechanisms developed by bacterial pathogens. In that context, several methods have been extensively used in recent years for the characterization of Mycobacterium tuberculosis PPI to further decipher TB pathogenesis. This review aims at compiling the most striking results based on in vivo methods (yeast and bacterial two-hybrid systems, protein complementation assays) for the specific study of PPI in mycobacteria. Moreover, newly developed methods, such as in-cell native mass resonance and proximity-dependent biotinylation identification, will have a deep impact on future mycobacterial research, as they are able to perform dynamic (transient interactions) and integrative (multiprotein complexes) analyses.

Subject Areas

tuberculosis; Mycobacterium; protein-protein interactions; virulence

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