Working Paper Article Version 1 This version is not peer-reviewed

Transcriptome and Metabolome Analysis Reveal Oral Secretions from Two Lepidoptera Species Induced Defense Response in Cotton

Version 1 : Received: 26 August 2019 / Approved: 27 August 2019 / Online: 27 August 2019 (10:06:32 CEST)

How to cite: Si, H.; Liu, H.; Sun, Y.; Xu, Z.; Liang, S.; LI, B.; Ding, X.; Li, J.; Wang, Q.; Sun, L.; Jin, S. Transcriptome and Metabolome Analysis Reveal Oral Secretions from Two Lepidoptera Species Induced Defense Response in Cotton. Preprints 2019, 2019080280 Si, H.; Liu, H.; Sun, Y.; Xu, Z.; Liang, S.; LI, B.; Ding, X.; Li, J.; Wang, Q.; Sun, L.; Jin, S. Transcriptome and Metabolome Analysis Reveal Oral Secretions from Two Lepidoptera Species Induced Defense Response in Cotton. Preprints 2019, 2019080280

Abstract

Cotton is one of the most important economic crops in the world, whereas insect attacks is a prime cause of its losses on yield and quality. Despite it is important, little is known about the mechanism of cotton response to insect. In this study, we simulated insect feeding by applying insect oral secretions (OS) to wounds, and combined transcriptome and metabolome analysis to comprehensively investigate how OS from two major pest species (Helicoverpa armigera and Spodoptera litura) affect cotton defense responses. Obvious differences were observed that 12,668 and 13,379 genes differently expressed respectively in cotton after being treated with different OS when compared with wounding along. Upon the addition of OS, JA-signaling pathway were rapidly and strongly induced, however SA-biosynthesis related gens were significantly down-regulated. By constructing the co-expression gene network, we identified a hub gene which encoding a leucine-rich repeat receptor kinase may play important role in recognition and signal transduction at early stage. We also found that OS from different insect species altered abundance of flavonoid related compound with different patterns. Interestingly, Gossypol which is a well-known anti-insect compound was kept a relative low content after OS application relative to wounding which implied the existence of the suppression effects of OS to cotton defense response. This study illustrated the transcriptional and metabolic changes of cotton in responding to OS from two chewing insect species, identified potential key gene during the interaction process and finds the evidence for OS’s inhibition effects on cotton defense response.

Subject Areas

Oral secretions; H. armigera; S. litura; Cotton; Suppression; Host plant defense

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