Preprint Article Version 1 This version is not peer-reviewed

Isolation and Culture of Skin-Derived Differentiated and Stem-Like Cells Obtained from the Arabian Camel (Camelus dromedarius)

Version 1 : Received: 8 May 2019 / Approved: 10 May 2019 / Online: 10 May 2019 (15:04:59 CEST)

How to cite: Saadeldin, I.; Swelum, A.; Noreldin, A.; Tukur, H.; Abdelazim, A.; Abomughaid, M.; Alowaimer, A. Isolation and Culture of Skin-Derived Differentiated and Stem-Like Cells Obtained from the Arabian Camel (Camelus dromedarius). Preprints 2019, 2019050135 (doi: 10.20944/preprints201905.0135.v1). Saadeldin, I.; Swelum, A.; Noreldin, A.; Tukur, H.; Abdelazim, A.; Abomughaid, M.; Alowaimer, A. Isolation and Culture of Skin-Derived Differentiated and Stem-Like Cells Obtained from the Arabian Camel (Camelus dromedarius). Preprints 2019, 2019050135 (doi: 10.20944/preprints201905.0135.v1).

Abstract

Elite camels often suffer from massive injuries. Thus, there is a pivotal need for a cheap and readily available regenerative medicine source. We isolated novel stem-like cells from camel skin and investigated their multipotency and resistance against various stresses. Skin samples were isolated from ears of five camels. Fibroblasts, keratinocytes, and spheroid progenitors were extracted. After separation of different cell lines by trypsinization, all cell lines were exposed to heat shock. Then, fibroblasts and dermal cyst-forming cells were examined under cryopreservation. Dermal cyst-forming cells were evaluated for resistance against osmotic pressure. The results revealed that resistance periods against trypsin were 1.5, 4, and 7 minutes for fibroblasts, keratinocytes, and spheroid progenitors, respectively. Furthermore, complete recovery of different cell lines after heat shock along with the differentiation of spheroid progenitors into neurons was observed. Fibroblasts and spheroid progenitors retained cell proliferation after cryopreservation. Dermal cyst-forming cells regained their normal structure after collapsing by osmotic pressure. The spheroid progenitors incubated in the adipogenic, osteogenic, and neurogenic media differentiated into the adipocytes, osteoblasts, and neurons, respectively. To the best of our knowledge, we isolated different unique cellular differons and stem-like cells from the camel skin and examined their multipotency for the first time.

Subject Areas

Skin; Cell culture; Stem cells; Differentiation; Camel

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