Preprint Article Version 1 This version is not peer-reviewed

FLYDE Plate: Open Source Anesthesia Labware for Drosophila Fly-Pushing!

Version 1 : Received: 4 May 2019 / Approved: 6 May 2019 / Online: 6 May 2019 (11:51:52 CEST)

How to cite: Hyde, J.; Long, T.A. FLYDE Plate: Open Source Anesthesia Labware for Drosophila Fly-Pushing!. Preprints 2019, 2019050060 (doi: 10.20944/preprints201905.0060.v1). Hyde, J.; Long, T.A. FLYDE Plate: Open Source Anesthesia Labware for Drosophila Fly-Pushing!. Preprints 2019, 2019050060 (doi: 10.20944/preprints201905.0060.v1).

Abstract

One of the most important pieces of equipment used in labs in culturing populations of fruit flies (Drosophila sp.) is that of the “CO2 gas plate”, which is used to anesthesize individuals during “fly-pushing”. This piece of equipment consists of a box with a porous top into which carbon-dioxide is pumped. Flies placed on its surface are left immobilized, permitting the sorting, categorizing and/or counting of flies during population culturing and experimental assays. Unfortunately, commercially available gas plates are typically expensive. Here, we describe a new design for a gas plate that can be easily produced using a 3D printer and a laser cutter, which we are making freely available to the fly community.

Supplementary and Associated Material

Subject Areas

open source; 3D printing; Drosophila; laser cutter; lab equipment; open labware; fly-pushing; fly pad; fly plate; CO2 anesthesia

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