Submitted:
19 April 2019
Posted:
23 April 2019
Read the latest preprint version here
Abstract
Subsidiary to detection and assignment of novel microRNAs in non-model taxa, it is standard to identify and compare genomic or transcript sequence of Drosha and Pasha. Detection of both (1) bona fide microRNAs and (2) presence of Drosha/Pasha orthologs is often assumed to represent a functional canonical eumetazoan microRNA biogenesis pathway. However, this is not often experimentally confirmed in non-model taxa, and therefore the assumption is not necessarily valid. Below I describe several lines of evidence for this assertion.
Keywords:
Microprocessor
; miRNA
; Drosha
; Pasha
; miRNA biogenesis pathway
; Holozoa
; Metazoa
; RNA interference
; bona fide miRNA
; canonical eumetazoan miRNA
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