Preprint Article Version 1 This version is not peer-reviewed

Susceptibility of Exopalaemon carinicauda to the Infection with Shrimp Hemocyte Iridescent Virus

Version 1 : Received: 12 February 2019 / Approved: 13 February 2019 / Online: 13 February 2019 (13:06:15 CET)

How to cite: Chen, X.; Qiu, L.; Wang, H.; Zou, P.; Dong, X.; Li, F.; Huang, J. Susceptibility of Exopalaemon carinicauda to the Infection with Shrimp Hemocyte Iridescent Virus. Preprints 2019, 2019020115 (doi: 10.20944/preprints201902.0115.v1). Chen, X.; Qiu, L.; Wang, H.; Zou, P.; Dong, X.; Li, F.; Huang, J. Susceptibility of Exopalaemon carinicauda to the Infection with Shrimp Hemocyte Iridescent Virus. Preprints 2019, 2019020115 (doi: 10.20944/preprints201902.0115.v1).

Abstract

In this study, ridgetail white prawns Exopalaemon carinicauda were infected per os with debris of Shrimp hemocyte iridescent virus (SHIV)-infected Penaeus vannamei and via intramuscular injection (im) with raw extracts of SHIV. The infected E. carinicauda showed obvious clinical symptoms, including weakness, empty gut and stomach, pale hepatopancreas, and partial death with cumulative mortality of (50.0±26.5)% and (76.7±18.3)%, respectively. Results of TaqMan probe based real-time quantitative PCR showed that the moribund and survival individuals with clinical signs of infected E. carinicauda were SHIV-positive. Histological examination showed that there were dark eosinophilic inclusions, of which some were surrounded with or contained tiny basophilic staining, and pyknosis in cytoplasm of hemocytes in the hepatopancreatic sinus, hematopoietic cells, and cuticular epithelium, etc. Positive hybridization signals were observed in stomach, hematopoietic tissue, cuticular epithelium, and hepatopancreatic sinus of infected prawns from both per os and im groups, according to the results of in situ DIG-labeling-loop-mediated DNA Amplification (ISDL). Transmission electron microscopy of ultrathin sections showed that icosahedral SHIV particles existed in hepatopancreatic sinus and gills of the infected E. carinicauda of the per os group. The viral particles were also observed in the hepatopancreatic sinus, gills, pereiopods, muscles, and uropods of the infected E. carinicauda from the im group. The assembled virions mostly distributed outside of the assembling area near cellular membrane of infected cells, which were with envelope and about 150 nm in diagonal diameter. The results of molecular biological tests, histopathological examination, ISDL, and transmission electron microscopy confirmed that E. carinicauda is one of the susceptible hosts of SHIV. This study also reminded that E. carinicauda showed some degree of tolerance to the infection with SHIV per os challenge mimicking natural pathway.

Subject Areas

Shrimp hemocyte iridescent virus; Exopalaemon carinicauda; susceptibility; host; ISDL

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