Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Exposure to Herbicides Primes P450-Mediated Detoxification of Helicoverpa armigera against Insecticide and Fungal Toxin

Zhongxiang Sun
ORCID logo ,
Cuicui Xu
,
Shi Chen
,
Qi Shi
,
Huanhuan Wang
,
Rumeng Wang
,
Yuanyuan Song
* ,
Rensen Zeng
*
Version 1 : Received: 26 December 2018 / Approved: 28 December 2018 / Online: 28 December 2018 (05:27:22 CET)

A peer-reviewed article of this Preprint also exists.

Sun, Z.; Xu, C.; Chen, S.; Shi, Q.; Wang, H.; Wang, R.; Song, Y.; Zeng, R. Exposure to Herbicides Prime P450-Mediated Detoxification of Helicoverpa armigera against Insecticide and Fungal Toxin. Insects 2019, 10, 28. Sun, Z.; Xu, C.; Chen, S.; Shi, Q.; Wang, H.; Wang, R.; Song, Y.; Zeng, R. Exposure to Herbicides Prime P450-Mediated Detoxification of Helicoverpa armigera against Insecticide and Fungal Toxin. Insects 2019, 10, 28.

Abstract

With the long-term and large-scale use, herbicides have been well known to influence tritrophic interactions particularly natural enemies of pests in agro-ecosystems. On the other hand, herbivorous insects, especially the generalist pests, have developed antagonistic interaction to different insecticides, toxic plant secondary metabolites and even heavy metals. However, whether exposure to herbicides would affect resistance of insects against insecticides is largely unknown, especially in agricultural pests. Here, we first reported that pre-exposure to two widely used herbicides butachlor and haloxyfop-methyl for 48 h can prime resistance of a generalist agricultural pest Helicoverpa armigera Hübner against insecticide methomyl and fungal toxin aflatoxin B1. In addition, there were no significant differences between control and herbicides-treated caterpillars on weight gain, pupal weight and pupation rates, suggesting that exposure to herbicides induce resistance of H. armigera accompanied with no fitness cost. Moreover, by determining detoxifying enzyme activities and toxicity bioassay with additional inhibitor of cytochrome P450 piperonyl butoxide (PBO), we showed that exposure to herbicides might prime P450-mediated detoxification of H. armigera against insecticide. Based on these results, we propose that exposure to herbicides primes resistance of H. armigera against insecticide by eliciting a clear elevation of predominantly P450 monooxygenase activities in midgut and fat body.

Keywords

herbicides; insecticides; antagonistic interaction; P450; Helicoverpa armiger

Subject

Biology and Life Sciences, Insect Science

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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