Preprint Article Version 1 This version is not peer-reviewed

Highly Sensitive Voltammetric Glucose Biosensor Based on Glucose Oxidase Encapsulated in a Chitosan/Kappa-Carrageenan/Gold Nanoparticle Bionanocomposite

Version 1 : Received: 2 December 2018 / Approved: 6 December 2018 / Online: 6 December 2018 (13:15:35 CET)

How to cite: Jaffrezic-Renault, N.; Rassas, I.; Braiek, M.; Bonhomme, A.; Bessueille, F.; Raffin, G.; Majdoub, H. Highly Sensitive Voltammetric Glucose Biosensor Based on Glucose Oxidase Encapsulated in a Chitosan/Kappa-Carrageenan/Gold Nanoparticle Bionanocomposite. Preprints 2018, 2018120085 Jaffrezic-Renault, N.; Rassas, I.; Braiek, M.; Bonhomme, A.; Bessueille, F.; Raffin, G.; Majdoub, H. Highly Sensitive Voltammetric Glucose Biosensor Based on Glucose Oxidase Encapsulated in a Chitosan/Kappa-Carrageenan/Gold Nanoparticle Bionanocomposite. Preprints 2018, 2018120085

Abstract

In this work, an enzymatic sensor, based on a bionanocomposite film consisting of a polyelectrolyte complex (PEC) [Chitosan/kappa-carrageenan] doped with gold nanoparticles (AuNPs) encapsulating glucose oxidase (GOD) deposited on a gold electrode (Au) for glucose sensing, is described. Using the electrocatalytic synergy of AuNPs and GOD as a model of enzyme, the variation of the current (µA) as a function of the log of the glucose concentration (log [glucose]), shows 3 times higher sensitivity for the modified electrode (283.9) compared to that of the PEC/GOD modified electrode (93.7), with a detection limit of about 5 µM and a linearity range between 10µM and 7mM. The response of the PEC/AuNPs/GOD based biosensor also presents good reproducibility, stability and negligible interfering effects from ascorbic acid, uric acid, urea and creatinine. The applicability of the PEC/AuNPs/GOD based biosensor was tested in glucose-spiked saliva samples and acceptable recovery rates were obtained.

Subject Areas

chitosan; kappa-carrageenan; polyelectrolyte complex; gold nanoparticles encapsulation; glucose oxidase; bionanocomposite.

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