He, L.; Xiao, J.; Rashid, K.Y.; Jia, G.; Li, P.; Yao, Z.; Wang, X.; Cloutier, S.; You, F.M. Evaluation of Genomic Prediction for Pasmo Resistance in Flax. Int. J. Mol. Sci.2019, 20, 359.
He, L.; Xiao, J.; Rashid, K.Y.; Jia, G.; Li, P.; Yao, Z.; Wang, X.; Cloutier, S.; You, F.M. Evaluation of Genomic Prediction for Pasmo Resistance in Flax. Int. J. Mol. Sci. 2019, 20, 359.
He, L.; Xiao, J.; Rashid, K.Y.; Jia, G.; Li, P.; Yao, Z.; Wang, X.; Cloutier, S.; You, F.M. Evaluation of Genomic Prediction for Pasmo Resistance in Flax. Int. J. Mol. Sci.2019, 20, 359.
He, L.; Xiao, J.; Rashid, K.Y.; Jia, G.; Li, P.; Yao, Z.; Wang, X.; Cloutier, S.; You, F.M. Evaluation of Genomic Prediction for Pasmo Resistance in Flax. Int. J. Mol. Sci. 2019, 20, 359.
Abstract
Pasmo (Septorialinicola) is a fungal disease causing major losses in seed yield and quality, and stem fibre quality in flax. Pasmo resistance (PR) is quantitative and has low heritability. To improve PR breeding efficiency, the accuracy of genomic prediction (GP) was evaluated using a diverse worldwide core collection of 370 accessions. Four marker sets, including three defined by 500, 134, and 67 previously identified quantitative trait loci (QTL) and one of 52,347 PR-correlated genome-wide single nucleotide polymorphisms, were used to build ridge regression best linear unbiased prediction (RR-BLUP) models using pasmo severity (PS) data collected from field experiments performed during five consecutive years. With five-fold random cross-validation, GP accuracy as high as 0.92 was obtained from the models using the 500 QTL when the average PS was used as the training dataset. GP accuracy increased with training population size, reaching values >0.9 with training population size greater than 185. Linear regression of the observed PS with the number of positive-effect QTL in accessions provided an alternative GP approach with an accuracy of 0.86. The results demonstrate the GP models based on marker information from all identified QTL and the 5-year PS average is highly effective for PR prediction.
Keywords
genomic selection; genomic prediction; genotyping by sequencing; pasmo resistance; pasmo severity; quantitative trait loci; single nucleotide polymorphism; Septoria linicola; flax
Subject
Biology and Life Sciences, Plant Sciences
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
