Preprint Article Version 1 This version is not peer-reviewed

Through the Looking Glass: Real Time Imaging in Brachypodium Roots and Osmotic Stress Analysis

Version 1 : Received: 17 November 2018 / Approved: 19 November 2018 / Online: 19 November 2018 (11:05:36 CET)

A peer-reviewed article of this Preprint also exists.

Khan, Z.; Karamahmutoğlu, H.; Elitaş, M.; Yüce, M.; Budak, H. THROUGH THE LOOKING GLASS: Real-Time Imaging in Brachypodium Roots and Osmotic Stress Analysis. Plants 2019, 8, 14. Khan, Z.; Karamahmutoğlu, H.; Elitaş, M.; Yüce, M.; Budak, H. THROUGH THE LOOKING GLASS: Real-Time Imaging in Brachypodium Roots and Osmotic Stress Analysis. Plants 2019, 8, 14.

Journal reference: Plants 2019, 8, 14
DOI: 10.3390/plants8010014

Abstract

To elucidate dynamic developmental processes in plants, live tissues and organs have to be visualized frequently and for long time periods. The development of roots is studied in depth at a cellular resolution not only to comprehend the basic processes fundamental to maintenance and pattern formation but also study stress tolerance adaptation in plants. Despite technological advancements, maintaining continuous access to samples and simultaneously preserving their morphological structures and physiological conditions without causing damage presents hindrances in the measurement, visualization and analyses of growing organs including plant roots. We propose a preliminary system which integrates the optical real-time visualization through light microscopy with a liquid culture which enables us to image at the tissue and cellular level horizontally growing Brachypodium roots every few minutes and up to 24 hours. We describe a simple setup which can be used to track the growth of the root as it grows including the root tip growth and osmotic stress dynamics. We demonstrate the system’s capability to scale down the PEG-mediated osmotic stress analysis and collected data on gene expression under osmotic stress.

Subject Areas

brachypodium; neutral red; root; casparian bands; PEG-6000; osmotic stress; real time imaging; PDMS

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