Preprint Article Version 1 This version is not peer-reviewed

Encapsidation of Different Plasmonic Gold Nanoparticles by the CCMV Capsid Protein

Version 1 : Received: 17 September 2018 / Approved: 17 September 2018 / Online: 17 September 2018 (09:54:48 CEST)

How to cite: Duran-Meza, A.L.; Escamilla-Ruiz, M.I.; Segovia-Gonzalez, X.F.; Villagrana-Escareño, M.V.; Vega-Acosta, J.R.; Ruiz-Garcia, J. Encapsidation of Different Plasmonic Gold Nanoparticles by the CCMV Capsid Protein. Preprints 2018, 2018090294 (doi: 10.20944/preprints201809.0294.v1). Duran-Meza, A.L.; Escamilla-Ruiz, M.I.; Segovia-Gonzalez, X.F.; Villagrana-Escareño, M.V.; Vega-Acosta, J.R.; Ruiz-Garcia, J. Encapsidation of Different Plasmonic Gold Nanoparticles by the CCMV Capsid Protein. Preprints 2018, 2018090294 (doi: 10.20944/preprints201809.0294.v1).

Abstract

Different types of gold nanoparticles have been synthesized that great potential in medical applications such as medical imaging, bio-analytical sensing and photothermal therapy. However, their stability, polydispersity and biocompatibility are major issues of concern. For example, the synthesis of gold nanorods, obtained through the elongated micelle process, produce them with a high positive surface charge that is cytotoxic. While gold nanoshells are unstable and within a few weeks they decompose due to Ostwald ripening. In this work, we report the self-assembly of the capsid protein of cowpea chlorotic mottle virus (CCMV) around spherical gold nanoparticles, gold nanorods and gold nanoshells to form virus-like particles (VLPs). All gold nanoparticles were synthesized or treated to give them a negative surface charge, so they can interact with the positive N-terminus of the capsid protein leading to the formation of the VLPs. To induce the protein self-assembly around the negative gold nanoparticles, we use different pH and ionic strength conditions that were determined from the capsid protein phase diagram. The encapsidation with the viral capsid protein confers them better biocompatibility, stability, monodispersity and a new biological substrate on which one can introduce specific ligands towards particular cells, broadening the possibilities of medical application.

Subject Areas

Plasmonic Nanoparticles; Gold Nanoparticles; Gold Nanorods; Gold Nanoshells; CCMV; Virus-Like Particles

Comments (0)

We encourage comments and feedback from a broad range of readers. See criteria for comments and our diversity statement.

Leave a public comment
Send a private comment to the author(s)
Views 0
Downloads 0
Comments 0
Metrics 0


×
Alerts
Notify me about updates to this article or when a peer-reviewed version is published.