Version 1
: Received: 17 September 2018 / Approved: 17 September 2018 / Online: 17 September 2018 (09:54:48 CEST)
How to cite:
Duran-Meza, A.L.; Escamilla-Ruiz, M.I.; Segovia-Gonzalez, X.F.; Villagrana-Escareño, M.V.; Vega-Acosta, J.R.; Ruiz-Garcia, J. Encapsidation of Different Plasmonic Gold Nanoparticles by the CCMV Capsid Protein. Preprints2018, 2018090294. https://doi.org/10.20944/preprints201809.0294.v1
Duran-Meza, A.L.; Escamilla-Ruiz, M.I.; Segovia-Gonzalez, X.F.; Villagrana-Escareño, M.V.; Vega-Acosta, J.R.; Ruiz-Garcia, J. Encapsidation of Different Plasmonic Gold Nanoparticles by the CCMV Capsid Protein. Preprints 2018, 2018090294. https://doi.org/10.20944/preprints201809.0294.v1
Duran-Meza, A.L.; Escamilla-Ruiz, M.I.; Segovia-Gonzalez, X.F.; Villagrana-Escareño, M.V.; Vega-Acosta, J.R.; Ruiz-Garcia, J. Encapsidation of Different Plasmonic Gold Nanoparticles by the CCMV Capsid Protein. Preprints2018, 2018090294. https://doi.org/10.20944/preprints201809.0294.v1
APA Style
Duran-Meza, A.L., Escamilla-Ruiz, M.I., Segovia-Gonzalez, X.F., Villagrana-Escareño, M.V., Vega-Acosta, J.R., & Ruiz-Garcia, J. (2018). Encapsidation of Different Plasmonic Gold Nanoparticles by the CCMV Capsid Protein. Preprints. https://doi.org/10.20944/preprints201809.0294.v1
Chicago/Turabian Style
Duran-Meza, A.L., J. Roger Vega-Acosta and Jaime Ruiz-Garcia. 2018 "Encapsidation of Different Plasmonic Gold Nanoparticles by the CCMV Capsid Protein" Preprints. https://doi.org/10.20944/preprints201809.0294.v1
Abstract
Different types of gold nanoparticles have been synthesized that great potential in medical applications such as medical imaging, bio-analytical sensing and photothermal therapy. However, their stability, polydispersity and biocompatibility are major issues of concern. For example, the synthesis of gold nanorods, obtained through the elongated micelle process, produce them with a high positive surface charge that is cytotoxic. While gold nanoshells are unstable and within a few weeks they decompose due to Ostwald ripening. In this work, we report the self-assembly of the capsid protein of cowpea chlorotic mottle virus (CCMV) around spherical gold nanoparticles, gold nanorods and gold nanoshells to form virus-like particles (VLPs). All gold nanoparticles were synthesized or treated to give them a negative surface charge, so they can interact with the positive N-terminus of the capsid protein leading to the formation of the VLPs. To induce the protein self-assembly around the negative gold nanoparticles, we use different pH and ionic strength conditions that were determined from the capsid protein phase diagram. The encapsidation with the viral capsid protein confers them better biocompatibility, stability, monodispersity and a new biological substrate on which one can introduce specific ligands towards particular cells, broadening the possibilities of medical application.
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
