Preprint Article Version 1 This version is not peer-reviewed

HS-SPME-GC-MS Analyses of Volatiles in Plant Populations – Quantitating Compound × Individual Matrix Effects

Version 1 : Received: 30 August 2018 / Approved: 30 August 2018 / Online: 30 August 2018 (21:22:47 CEST)

A peer-reviewed article of this Preprint also exists.

Burzynski-Chang, E.A.; Ryona, I.; Reisch, B.I.; Gonda, I.; Foolad, M.R.; Giovannoni, J.J.; Sacks, G.L. HS-SPME-GC-MS Analyses of Volatiles in Plant Populations—Quantitating Compound × Individual Matrix Effects. Molecules 2018, 23, 2436. Burzynski-Chang, E.A.; Ryona, I.; Reisch, B.I.; Gonda, I.; Foolad, M.R.; Giovannoni, J.J.; Sacks, G.L. HS-SPME-GC-MS Analyses of Volatiles in Plant Populations—Quantitating Compound × Individual Matrix Effects. Molecules 2018, 23, 2436.

Journal reference: Molecules 2018, 23, 2436
DOI: 10.3390/molecules23102436

Abstract

Headspace solid phase microextraction (HS-SPME) coupled to gas chromatography-mass spectrometry is widely employed for volatile analyses of plants, including mapping populations used in plant breeding research. Studies often employ a single internal surrogate standard, even when multiple analytes are measured, with the assumption that any relative changes in matrix effects among individuals would be similar for all compounds, i.e. matrix effects do not show Compound × Individual interactions. We tested this assumption using individuals from two plant populations, an interspecific grape (Vitis spp.) mapping population (n = 140) and a tomato (Solanum spp.) recombinant inbred line (RIL) population (n = 148). Individual plants from the two populations were spiked with a cocktail of internal standards (n = 6, 9, respectively) prior to HS-SPME-GC-MS. Variation in the relative responses of internal standards indicated that Compound × Individual interactions exist but were different between the two populations. For the grape population, relative responses among pairs of internal standards varied considerably among individuals, with a maximum of 249% relative standard deviation (RSD) for the pair of [U13C]hexanal and [U13C]hexanol. However, in the tomato population, relative responses of internal standard pairs varied much less, with pairwise RSDs ranged from 8% to 56%. The approach described in this paper could be used to evaluate the suitability of using surrogate standards for HS-SPME-GC-MS studies in other plant populations.

Subject Areas

Breeding population, internal standards, matrix effects, plant volatiles, SPME, odorant analysis

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