Preprint Article Version 1 This version is not peer-reviewed

A Rapid, Sensitive, and Portable Biosensor Assay for the Detection of Botulinum Neurotoxin Serotype A in Complex Food Matrices

Version 1 : Received: 21 August 2018 / Approved: 22 August 2018 / Online: 22 August 2018 (04:59:24 CEST)

A peer-reviewed article of this Preprint also exists.

Tam, C.C.; Flannery, A.R.; Cheng, L.W. A Rapid, Sensitive, and Portable Biosensor Assay for the Detection of Botulinum Neurotoxin Serotype A in Complex Food Matrices. Toxins 2018, 10, 476. Tam, C.C.; Flannery, A.R.; Cheng, L.W. A Rapid, Sensitive, and Portable Biosensor Assay for the Detection of Botulinum Neurotoxin Serotype A in Complex Food Matrices. Toxins 2018, 10, 476.

Journal reference: Toxins 2018, 10, 476
DOI: 10.3390/toxins10110476

Abstract

Botulinum neurotoxin (BoNT) intoxication can lead to the disease botulism, characterized by flaccid muscle paralysis that can cause respiratory failure and death.  Due to the significant morbidity and mortality costs associated with BoNTs high toxicity, developing highly sensitive, rapid, and field-deployable assays are critically important to protect the nation’s food supply against either accidental or intentional contamination. We report here that the B-cell based biosensor assay (CANARY® Zephyr) detects BoNT/A in buffer and various food matrices rapidly in ≤ 40 min, in small volumes ≈ 50 μL, with minimal processing of samples, and is extremely portable (suitcase-sized equipment). BoNT/A was detected at limits of detection (LOD) < 0.075 ng ± 0.02 in assay buffer while milk matrices (non-fat, 2 %, whole milk) increased the LOD to < 0.175 – 0.314 ng. Limits of detection for the assay in complex foods were < 1 ng ± 0.0 (neutralized acidic juices-carrot, orange and apple); < 16.7 ng ± 7.7 (liquid egg); and varied from < 0. 39 – 3.125 ng for solid complex foods (ground beef, green bean baby puree, smoked salmon). These results show that the CANARY® Zephyr assay can be a highly useful tool in clinical, environmental, and food safety surveillance programs.

Subject Areas

botulinum neurotoxin; biosensor; CANARY®, detection; B-cell based assay; immunoassay; food matrices

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