Preprint Article Version 1 This version is not peer-reviewed

Histological and Immunohistochemical Effects of Neuroectodermal Cells Transplantation after Spinal Cord Injury in Rats

Version 1 : Received: 1 August 2018 / Approved: 2 August 2018 / Online: 2 August 2018 (06:26:46 CEST)

How to cite: Al-Karim, S.; Ramadan, W.S.; Abdel-Hamid, G.A.; Al Qudsi, F. Histological and Immunohistochemical Effects of Neuroectodermal Cells Transplantation after Spinal Cord Injury in Rats. Preprints 2018, 2018080035 (doi: 10.20944/preprints201808.0035.v1). Al-Karim, S.; Ramadan, W.S.; Abdel-Hamid, G.A.; Al Qudsi, F. Histological and Immunohistochemical Effects of Neuroectodermal Cells Transplantation after Spinal Cord Injury in Rats. Preprints 2018, 2018080035 (doi: 10.20944/preprints201808.0035.v1).

Abstract

Background: In spinal cord injury, radical treatment is still a persistent hope for patients and clinicians. Our study aimed to determine the different histological changes in central, cranial and caudal sites of compressed spinal cord as a result of neuroectodermal stem cells (NESCs) transplantation in rats. Material and methods: For extraction of NESCs, future brains were extracted from mice embryos (10-days old) and cultured.  Eighty, male rats were divided randomly into control, sham (20 rats each); while 40 rats were subjected to compressed spinal cord injury (CSCI). Seven days after spinal cord injury, rats were subdivided into 2 groups (20 rats each); an untreated and treated with NESCs injected cranial and caudal to the site of the spinal cord injury. Rats were sacrificed 4 weeks after transplantations of NESCs and specimens from the spinal cord at the central, cranial and caudal to site of spinal cord injury were proceeded to be stained with haematoxylin & eosin, osmic acid and Immunohistochemistry of glial fibrillary acidic protein (GFAP). Results: Sections of CSCI revealed areas of hemorrhages, necrosis and cavitation limited by reactive astrocytosis, with upregulation of GFAP expression. Evidence of remyelination and mitigation of histopathological features, reactive astrocytosis in CSCI sections were more pronounced in cranial than in caudal region. Conclusions: NESCs transplantation ameliorated the pathological changes, promoted remyelination.

Subject Areas

neural; stem cells; compressed; spinal cord; injury; regeneration; rat

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