Preprint Article Version 1 This version is not peer-reviewed

Factors Affecting Mass Transport Properties of Poly(Ε-Caprolactone) Membranes for Tissue Engineering Bioreactors

Version 1 : Received: 20 July 2018 / Approved: 20 July 2018 / Online: 20 July 2018 (14:01:37 CEST)

A peer-reviewed article of this Preprint also exists.

Diban, N.; Gómez-Ruiz, B.; Lázaro-Díez, M.; Ramos-Vivas, J.; Ortiz, I.; Urtiaga, A. Factors Affecting Mass Transport Properties of Poly(ε-caprolactone) Membranes for Tissue Engineering Bioreactors. Membranes 2018, 8, 51. Diban, N.; Gómez-Ruiz, B.; Lázaro-Díez, M.; Ramos-Vivas, J.; Ortiz, I.; Urtiaga, A. Factors Affecting Mass Transport Properties of Poly(ε-caprolactone) Membranes for Tissue Engineering Bioreactors. Membranes 2018, 8, 51.

Journal reference: Membranes 2018, 8, 51
DOI: 10.3390/membranes8030051

Abstract

High porosity and mass transport properties of microfiltration polymeric membranes benefits nutrients supply to cells when used as scaffolds in interstitial perfusion bioreactors for tissue engineering. High nutrients transport is assumed when pore size and porosity of the membrane are in the micrometric range. The present work demonstrates that the study of membrane fouling by proteins present in the culture medium, though not done usually, should be included in the routine testing of new polymer membranes for this intended application. Two poly(ε-caprolactone) microfiltration membranes presenting similar average pore size (~0.7µm) and porosity (>80%) but different external surface porosity and pore size have been selected as case study. The present work demonstrates that a membrane with lower surface pore abundance and smaller external pore size (~0.67 µm), combined with adequate hydrodynamics and tangential flow filtration mode is usually more convenient to guarantee high flux of nutrients. On the contrary, having large external pore size (~1.70µm) and surface porosity would incur in important internal protein fouling that could not been prevented with the operation mode and hydrodynamics of the perfusion system. Additionally, the use of glycerol in the drying protocols of the membranes might cause plasticization and a consequent reduction of mass transport properties due to membrane compaction by the pressure exerted to force perfusion. Therefore, preferentially, drying protocols that omit the use of plasticizing agents are recommended.

Subject Areas

membrane fouling; membrane plasticization; nutrients transport properties; perfusion bioreactors; tissue engineering

Comments (0)

We encourage comments and feedback from a broad range of readers. See criteria for comments and our diversity statement.

Leave a public comment
Send a private comment to the author(s)
Views 0
Downloads 0
Comments 0
Metrics 0


×
Alerts
Notify me about updates to this article or when a peer-reviewed version is published.